Experience with Danish mix-ELISA in the United States

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2001-01-01
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Daniels, Michael
Zhang, Yuhua
Erdman, Matthew
Turney Harris, Isabel
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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This paper details some of our experiences with Danish mix-ELISA (DME) testing on herds in the United States. In contrast to Denmark, clinical outbreaks of Salmonella Choleraesuis occur in the U.S. We examine the appropriateness of the current cut-off of OD%>=40 for U.S. herds by examining serum and fecal samples collected from individua l pigs and tested with the DME and culture, respectively. We report the estimated sensitivity and specificity of the DME using the original and possibly modified cut-off values. The 30% cutoff was deemed optimal with a sensitivity of .57 (.45,.82) and a specificity of .84 (.68,.98) for the first set of samples and .69(.49,.93) and .63(.53,.76) for the second set of samples. A major use of these tests is for monitoring herds for Salmonella exposure over time. Information on the sensitivity and specificity of the DME is helpful in determining how many animals in a herd to sample and how often.

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