Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Thumbnail Image
Supplemental Files
Date
2009-11-09
Authors
Simons, Andrean
Renouf, Mathieu
Murphy, Patricia
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Person
Hendrich, Suzanne
University Professor Emeritus
Research Projects
Organizational Units
Organizational Unit
Journal Issue
Is Version Of
Versions
Series
Abstract

-OH-flavonoids disappeared more rapidly from human fecal incuba- tions and were less absorbable by humans than flavonoids without 5-OH moieties. Anaerobic fecal disappearance rates over 24 h were determined for 15 flavonoids in samples from 20 men and 13 women. In these anaerobic fecal mixtures, flavonoids with 5,7,40-OH groups, genistein, apigenin, naringenin, luteolin, kaempferol, and quercetin (disappearance rate, k = 0.46 ( 0.10 h-1), and methoxylated flavonoids, hesperetin and glycitein (k = 0.24 ( 0.21 h-1), disappeared rapidly compared with flavonoids lacking 5-OH (e.g., daidzein, k = 0.07 ( 0.03 h-1). Apparent absorption of flavonoids that disappeared rapidly from in vitro fecal incubations, genistein, naringenin, quercetin, and hesperetin, was compared with that of daidzein, a slowly disappearing flavonoid, in 5 men and 5 women. Subjects ingested 104 μmol of genistein and 62 μmol of daidzein (soy milk), 1549 μmol of naringenin and 26 μmol of hesperetin (grapefruit juice), and 381 μmol of quercetin (onions) in three test meals, each separated by 1 week. Blood and urine samples were collected over 24 h after each test meal. Plasma flavonoid concentrations ranged from 0.01 to 1 μM. The apparent absorption, expressed as percentage of ingested dose excreted in urine, was significantly less for naringenin (3.2 ( 1.7%), genistein (7.2 ( 4.6%), hesperetin (7.3 ( 3.2%), and quercetin (5.6 ( 3.7%) compared with daidzein (43.4 ( 15.5%, p = 0.02). These data affirmed the hypothesis that the 5,7,40-OH of flavonoids limited apparent absorption of these compounds in humans.

Comments

Reprinted with permission from Journal of Agricultural and Food Chemistry,58(1)2009: 141-147. doi: 10.1021/jf902284u. Copyright 2009 American Chemical Society.

Description
Keywords
Citation
DOI
Copyright
Thu Jan 01 00:00:00 UTC 2009
Collections