Interaction of Bifidobacterium animalis Subspecies lactis (Bb12) and Salmonella typhimurium in Continuous-Flow Chemostatic Culture

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2007-01-01
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Harvey, R.
Genovese, K.
Droleskey, R.
Andrews, K.
Solano-Aguilar, G.
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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A commercially available probiotic, Bifidobactenum animalis subspecies lactis (Bb12) was adapted to and maintained in a continuous-flow chemostat culture. We evaluated the growth characteristics and mteractive effects of Bb12 and Salmonella typhimurium (St) when cultivated singly or together. When the continuous-now culture of Bb12 was challenged with 104 to 107 CFU/ml of St, the St was eliminated within 24 h. This was replicated 3 times. Because the pH of the Bb12 was 4.5, it appeared that St elimination was due to the reduced pH. In a second study, St was grown in pure culture and the pH reduced to 4.5. Although still present, St concentrations dropped to unculturable levels within 28 h. In a third study, the pH of the Bb12 culture was maintained at pH 5.6 by means of a continuous drip of NaOH and challenged with St. Although at reduced concentrations (1.03 CFU/ml), the St remained in the chemostat until day 9 when the drip was discontinued By day 14, the St was elimmated. It is apparent in these m vitro studies that Bb12 has antagonistic properties against St and it is possible that there could be some in vivo applications of Bb 12 against St.

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Mon Jan 01 00:00:00 UTC 2007