DND1 Expression and Function in the Porcine Ovary, Oocyte and Embryo

dc.contributor.author Yang, Cai-Xia
dc.contributor.author Wright, Elane
dc.contributor.author Ross, Jason
dc.date 2018-08-25T21:56:04.000
dc.date.accessioned 2020-06-29T23:34:09Z
dc.date.available 2020-06-29T23:34:09Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.issued 2012-01-01
dc.description.abstract <p>DND1 (dead end homolog 1), belonging to the RNA binding protein family, can impact miRNA:mRNA functional pathway and in turn may contribute to maintaining normal oocyte growth and quality as well as embryo development following fertilization. To characterize DND1 in pig maturing oocytes and cumulus cells, and early embryos, we examined DND1 mRNA and protein expression using quantitative RT-PCR, Western blot and immunostaining. We found: (1) DND1 protein is expressed during pig follicle development; (2) DND1 is dynamically expressed at both mRNA and protein level in the maturing oocyte and early in vitro fertilized embryos; (3) DND1 mRNA is expressed in cumulus cells surrounding the maturing oocyte; and (4) DND1 protein is localized in the cytoplasm of pig maturing oocytes and early embryos. Our work provides useful data for functional study of DND1 proteins in female gametogenesis and developing embryos, which will benefit animal reproduction health and provides foundational knowledge for improving swine reproductive efficiency.</p>
dc.identifier archive/lib.dr.iastate.edu/ans_air/vol658/iss1/72/
dc.identifier.articleid 1778
dc.identifier.contextkey 3410210
dc.identifier.doi https://doi.org/10.31274/ans_air-180814-1372
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_air/vol658/iss1/72
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/8913
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R2740
dc.source.bitstream archive/lib.dr.iastate.edu/ans_air/vol658/iss1/72/R2740.pdf|||Sat Jan 15 01:43:45 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R2740
dc.title DND1 Expression and Function in the Porcine Ovary, Oocyte and Embryo
dc.type article
dc.type.genre swine
dspace.entity.type Publication
relation.isJournalIssueOfPublication 6dc63976-6a4a-4b75-a79f-e40937b79e61
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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