Human Brain Endothelial Cell Seeded on Inner Surface of Alginate Hollow Microfibers

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Aykar, Saurabh S.
Alimoradi, Nima
Petersen, Isaac S.
Montazami, Reza
Brockman, Amanda L.
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Hashemi, Nicole
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Mechanical Engineering
The Department of Mechanical Engineering at Iowa State University is where innovation thrives and the impossible is made possible. This is where your passion for problem-solving and hands-on learning can make a real difference in our world. Whether you’re helping improve the environment, creating safer automobiles, or advancing medical technologies, and athletic performance, the Department of Mechanical Engineering gives you the tools and talent to blaze your own trail to an amazing career.
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The Office of Biotechnology facilitates and advances programs in research, education, and outreach that contribute to the goals of Iowa State University’s Strategic Plan in the area of biotechnology. The Office oversees the biotechnology programs developed by the university’s Biotechnology Council and the Office of the Vice President for Research. The Office of Biotechnology works with the university’s biotechnology faculty and administrators to ensure effectiveness in research, education, and technology transfer related to the application of molecular biology to the development of useful products and processes.
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Barrier functionality of the blood-brain barrier (BBB) is provided by the tight junctions formed by a monolayer of the human brain endothelial cells (HBECs) internally around the blood capillaries. To mimic such barrier functionality in vitro, replicating the hollow tubular structure of the BBB along with the HBECs monolayer on its inner surface is crucial. Here, we developed an invasive microfluidic technique to obtain the HBECs monolayer on the inner surface of alginate-based hollow microfibers. The HBECs were seeded on the inner surface of these microfibers using a custom-built microfluidic device. The seeded HBECs were monitored for 9 days after manufacturing and cultured to form a monolayer on the inner surface of the alginate hollow microfibers in the maintenance media. A higher cell seeding density of 217 cells/mm length of the hollow microfiber was obtained using our microfluidic technique. Moreover, high accuracy of around 96 % was obtained in seeding cells on the inner surface of alginate hollow microfibers. The microfluidic method illustrated in this study could be extrapolated to obtain a monolayer of different cell types on the inner surface of alginate hollow microfibers with cell-compatible ECM matrix proteins. Furthermore, it will enable us to mimic a range of microvascular systems in vitro by closely replicating the structural attributes of the native structure.
This article is published as Aykar, Saurabh S., Nima Alimoradi, Isaac S. Petersen, Reza Montazami, Amanda L. Brockman, and Nicole N. Hashemi. "Human Brain Endothelial Cell Seeded on Inner Surface of Alginate Hollow Microfibers." bioRxiv (2023). DOI: 10.1101/2023.01.04.522758. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Posted with permission.
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