Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables

dc.contributor.author Jarboe, Laura
dc.contributor.author Khakbaz, Pouyan
dc.contributor.author Chen, Yingxi
dc.contributor.author Lombardo, Jeremy
dc.contributor.author Yoon, Jong Moon
dc.contributor.author Shanks, Jacqueline
dc.contributor.author Klauda, Jeffery B.
dc.contributor.author Jarboe, Laura B.
dc.contributor.department Chemical and Biological Engineering
dc.contributor.department Microbiology
dc.contributor.department Bioinformatics and Computational Biology
dc.contributor.department NSF Engineering Research Center for Biorenewable Chemicals
dc.date.accessioned 2022-02-15T22:14:02Z
dc.date.available 2022-02-15T22:14:02Z
dc.date.issued 2017-11
dc.description.abstract Economically competitive microbial production of biorenewable fuels and chemicals is often impeded by toxicity of the product to the microbe. Membrane damage is often identified as a major mechanism of this toxicity. Prior efforts to strengthen the microbial membrane by changing the phospholipid distribution have largely focused on the fatty acid tails. Herein, a novel strategy of phospholipid head engineering is demonstrated in Escherichia coli. Specifically, increasing the expression of phosphatidylserine synthase (+pssA) was found to significantly increase both the tolerance and production of octanoic acid, a representative membrane-damaging solvent. Tolerance of other industrially-relevant inhibitors, such as furfural, acetate, toluene, ethanol and low pH was also increased. In addition to the increase in the relative abundance of the phosphoethanolamine (PE) head group in the +pssA strain, there were also changes in the fatty acid tail composition, resulting in an increase in average length, percent unsaturation and decreased abundance of cyclic rings. This +pssA strain had significant changes in: membrane integrity, surface potential, electrochemical potential and hydrophobicity; sensitivity to intracellular acidification; and distribution of the phospholipid tails, including an increase in average length and percent unsaturation and decreased abundance of cyclic rings. Molecular dynamics simulations demonstrated that the +PE membrane had increased resistance to penetration of ethanol into the hydrophobic core and also the membrane thickness. Further hybrid models in which only the head group distribution or fatty acid tail distribution was altered showed that the increase in PE content is responsible for the increase in bilayer thickness, but the increased hydrophobic core thickness is due to altered distribution of both the head groups and fatty acid tails. This work demonstrates the importance of consideration of the membrane head groups, as well as a modeling approach, in membrane engineering efforts.
dc.description.comments This is a manuscript of an article published as Tan, Zaigao, Pouyan Khakbaz, Yingxi Chen, Jeremy Lombardo, Jong Moon Yoon, Jacqueline V. Shanks, Jeffery B. Klauda, and Laura R. Jarboe. "Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables." Metabolic Engineering 44 (2017): 1-12. DOI: 10.1016/j.ymben.2017.08.006. Copyright 2017 International Metabolic Engineering Society. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0). Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/7vdXPZGv
dc.language.iso en_US
dc.publisher Elsevier Inc.
dc.source.uri https://doi.org/10.1016/j.ymben.2017.08.006 *
dc.subject.disciplines DegreeDisciplines::Engineering::Chemical Engineering::Biochemical and Biomolecular Engineering
dc.subject.disciplines DegreeDisciplines::Physical Sciences and Mathematics::Environmental Sciences::Oil, Gas, and Energy
dc.subject.disciplines DegreeDisciplines::Engineering::Chemical Engineering::Membrane Science
dc.title Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
relation.isOrgUnitOfPublication 76818945-1d8c-461a-bbac-df615c052c9e
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
relation.isOrgUnitOfPublication 0cfd3a08-6d57-4971-8cfd-5a3a190929bc
File
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2017-JarboeLaura-EngineeringEscherichia.pdf
Size:
1.64 MB
Format:
Adobe Portable Document Format
Description:
Collections