Biochemical evaluation of germplasm regeneration methods for cucumber, Cucumis sativus L. Widrlechner, Mark Knerr, Larry Staub, Jack Widrlechner, Mark Reitsma, Kathleen
dc.contributor.department Agronomy
dc.contributor.department North Central Regional Plant Introduction Station 2018-02-18T09:12:01.000 2020-06-30T06:10:55Z 2020-06-30T06:10:55Z 1992
dc.description.abstract <p>During the 1980s, the North Central Regional Plant Introduction Station replaced open-pollinated samples of Cucumis sativus with samples regenerated by cage pollination. Enzyme polymorphisms, detected by horizontal starch gel electrophoresis, were evaluated to document genetic changes in 157 Cucumis accessions resulting from changes in seed-regeneration methods. Analysis of levels of sample homozygosity indicates that cage-pollinated samples are significantly more homozygous than the open-pollinated samples they replaced, but the two groups of samples differ little in overall allelic composition. The frequency of rare alleles also has not changed significantly, although three alleles were found in cage-pollinated samples that were not noted in their older counterparts. The results of this study may not be interpreted as conclusive support for the use of cage pollination for regeneration of Cucumis germplasm collections. But the comparison of within-accession levels of homozygosity suggests that the genetic integrity of individual accessions is maintained better with cage pollination than with less-controlled regeneration methods. Only a genetic comparison of original seed samples with regenerated samples can conclusively document the effectiveness of any regeneration system.</p>
dc.description.comments <p>This article is from <em>FAO/IBPGR Plant Genetic Resources Newsletter </em>88/89 (1992): 1.</p>
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dc.identifier archive/
dc.identifier.articleid 1063
dc.identifier.contextkey 10042230
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ncrpis_pubs/52
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 00:47:04 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Sciences
dc.title Biochemical evaluation of germplasm regeneration methods for cucumber, Cucumis sativus L.
dc.type article
dc.type.genre article
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