Clinical and Pathologic Comparison of Acute Leptospirosis in Dogs Caused by Two Strains of Leptospirosis Kirschneri Serovar Grippotyphosa

dc.contributor.author Greenlee, Justin
dc.contributor.author Andreasen, Claire
dc.contributor.author Bolin, Carole
dc.contributor.author Alt, David
dc.contributor.author Cheville, Norman
dc.contributor.author Andreasen, Claire
dc.contributor.department Veterinary Pathology
dc.date 2018-02-16T19:37:58.000
dc.date.accessioned 2020-07-07T05:16:05Z
dc.date.available 2020-07-07T05:16:05Z
dc.date.issued 2004-08-01
dc.description.abstract <p>Objective—To develop a method for inducing acute leptospirosis in dogs. Animals—31 nine-week-old female Beagles. Procedure—Beagles were randomly assigned to 2 inoculation groups or a control group. Dogs were inoculated on 3 successive days by conjunctival instillation of 5 X 107 cells of Leptospira kirschneri serovar grippotyphosa strain 82 (12 dogs) or strain RM 52 (14 dogs). Control dogs (n = 5) were similarly inoculated with sterile leptospiral culture media. Clinical signs, clinicopathologic variables, anti-leptospiral antibody titers, and evidence of leptospires in tissues and body fluids were evaluated. Dogs were euthanatized and necropsied on days 7, 14, 22, or 28 after inoculation or as required because of severe illness. Results—Clinical signs in infected dogs included conjunctivitis, lethargy, diarrhea, dehydration, vomiting, and icterus. Consistent clinicopathologic alterations included azotemia, hyperphosphatemia, increased anion gap, hyperbilirubinemia, and an increase in alkaline phosphatase activity. Leptospires were cultured from the kidneys (11/12), urine (6/9), aqueous humor (9/12), blood (12/12), and liver (12/12) of dogs inoculated with strain 82. Only 3 of 14 dogs became infected after inoculation with strain RM 52. Histopathologic lesions in infected dogs included interstitial nephritis, renal tubular degeneration and necrosis, pulmonary hemorrhage, and hepatic edema and perivasculitis. Conclusions and Clinical Relevance—Conjunctival exposure to L kirschneri serovar grippotyphosa strain 82 resulted in acute leptospirosis in all inoculated dogs, but only 3 of 14 dogs inoculated with strain RM 52 became infected. This method of infection by serovar grippotyphosa can be used to study the pathogenesis and prevention of leptospirosis in dogs.</p>
dc.description.comments <p>This article is from <em>American Journal of Veterinary Research</em> 65 (2004): 1100.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/70/
dc.identifier.articleid 1068
dc.identifier.contextkey 7356569
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/70
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92498
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/70/2004_Andreasen_ClinicalPathologic.pdf|||Sat Jan 15 01:40:18 UTC 2022
dc.subject.disciplines Small or Companion Animal Medicine
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords acute leptospirosis
dc.subject.keywords conjunctival exposure
dc.subject.keywords serovar
dc.subject.keywords canicola
dc.subject.keywords icterohaemorrhagiae
dc.title Clinical and Pathologic Comparison of Acute Leptospirosis in Dogs Caused by Two Strains of Leptospirosis Kirschneri Serovar Grippotyphosa
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a7eb4f05-0652-4bef-9192-78762d4cc3f0
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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