On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation

Yang, Renyuan
Boldrey, Joseph
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Chemical and Biological EngineeringGenetics, Development and Cell BiologyElectrical and Computer EngineeringMaterials Science and EngineeringAmes Laboratory
Glial cell-derived neurotrophic factor (GDNF) is a small protein potently promoting the survival of dopaminergic and motor neurons. GDNF can be secreted from different types of cells including the dopaminergic neural cell line, N27. N27 cells, a rat dopaminergic neural cell line, is regarded as a suitable in vitro model for Parkinson's disease (PD) research. For PD treatment, transcranial magnetic stimulation (TMS), a noninvasive therapeutic method, showed beneficial clinical effects, but the mechanism for its benefit is not understood. Because GDNF is a potent neurotrophic factor, it is of great value to evaluate if GDNF secretion from N27 cells can be affected by magnetic stimulation (MS). However, the current methods for detecting GDNF are time-consuming and expensive. In this paper we outline the detection of GDNF secretion from N27 cells by ultrasensitive nanopore thin film sensors (nanosensor) for the first time. As low as 2 pg/mL GDNF can be readily detected by the nanosensor. Furthermore, we show that MS can promote GDNF secretion from N27 cells. Specifically, the GDNF concentration in N27 cell-conditioned media under MS treatment shows statistically significant increase up to 2-fold after 5 days in vitro in comparison with the control. This nanosensor along with the in vitro PD model N27 cells provides a low-cost, easy-to-use, sensitive approach for studying potential cell biological mechanisms of the clinical benefits of MS on PD.
This is a manuscript of an article published as Yang, Renyuan, Joseph Boldrey, David Jiles, Ian Schneider, and Long Que. "On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation." Biosensors and Bioelectronics 182 (2021): 113179. DOI: 10.1016/j.bios.2021.113179. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0). Copyright 2021 Elsevier B.V.. Posted with permission.
Dopaminergic cells, Transcranial magnetic stimulation, Cell secretion, Nanopore thin film sensor, Neurotrophic factors detection