Evaluation of different enrichment media for the isolation of Salmonella spp from faeces and lymph nodes in slaughter pigs

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2001-01-01
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Nollet, Nathalie
Maes, Dominiek
De Zutter, Lieven
de Kruif, Aart
van Hoof, Jan
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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The aim of the study was to compare the isolation results of Salmonella spp. from faeces and mesenterial lymph nodes on 2 semi-solid media and 2 broths. For the faeces samples, Modified Semi-solid Rappaport Vassiliadis (MSRV) seemed to be the medium with the highest sensitivity. For the lymph node tissue samples, there was no significant difference between the different media. In parallel testing, for both types of sample a combination of RV or IT with MSRV seemed to be the best although differences were small. The use of such a combination is important because of the possible differences in motility characteristics among different Salmonella serotypes. Another goal was the evaluation of different sizes of sample material. To increase the sensitivity, it is important to use enough sample material. There was a significant difference regarding the number of positives in lymph node samples and in faeces samples, so not every carrier animal appeared to shed Salmonella or vice versa.

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