The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca

dc.contributor.author Winkelman, Dirk C.
dc.contributor.author Nikolau, Basil
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Center for Metabolic Biology
dc.date.accessioned 2024-04-04T18:31:14Z
dc.date.available 2024-04-04T18:31:14Z
dc.date.issued 2022-06-01
dc.description.abstract The aerobic, thermophilic Actinobacterium, Thermobifida fusca has been proposed as an organism to be used for the efficient conversion of plant biomass to fatty acid-derived precursors of biofuels or biorenewable chemicals. Despite the potential of T. fusca to catabolize plant biomass, there is remarkably little data available concerning the natural ability of this organism to produce fatty acids. Therefore, we determined the fatty acids that T. fusca produces when it is grown on different carbon sources (i.e., glucose, cellobiose, cellulose and avicel) and at two different growth temperatures, namely at the optimal growth temperature of 50°C and at a suboptimal temperature of 37°C. These analyses establish that T. fusca produces a combination of linear and branched chain fatty acids (BCFAs), including iso-, anteiso-, and 10-methyl BCFAs that range between 14- and 18-carbons in length. Although different carbon sources and growth temperatures both quantitatively and qualitatively affect the fatty acid profiles produced by T. fusca, growth temperature is the greater modifier of these traits. Additionally, genome scanning enabled the identification of many of the fatty acid biosynthetic genes encoded by T. fusca.
dc.description.comments This article is published as Winkelman DC and Nikolau BJ (2022) The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca. Front. Mol. Biosci. 9:896226. doi: 10.3389/fmolb.2022.896226.<br/><br/>© 2022 Winkelman and Nikolau. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/jrl8K06r
dc.language.iso en
dc.publisher Frontiers Media
dc.source.uri https://doi.org/10.3389/fmolb.2022.896226 *
dc.subject.disciplines DegreeDisciplines::Life Sciences::Biochemistry, Biophysics, and Structural Biology
dc.subject.keywords Thermobifida fusca
dc.subject.keywords Actinomycete
dc.subject.keywords fatty acid biosynthesis pathway
dc.subject.keywords principal component analysis
dc.subject.keywords gas chromatography- mass spectrometry
dc.subject.keywords branched chain fatty acids
dc.subject.keywords fatty acid synthase
dc.title The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
dc.type Article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
relation.isOrgUnitOfPublication 6071c08a-f86e-4e98-8a57-1c936baef5e5
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