Novel Combined Tissue Transcriptome Analysis After Lentogenic Newcastle Disease Virus Challenge in Inbred Chicken Lines of Differential Resistance Deist, Melissa Gallardo, Rodrigo Dekkers, Jack Zhou, Huaijun Lamont, Susan
dc.contributor.department Animal Science 2020-09-11T22:06:04.000 2021-02-24T21:10:58Z 2021-02-24T21:10:58Z Wed Jan 01 00:00:00 UTC 2020 2020-02-04
dc.description.abstract <p>Disease has large negative impacts on poultry production. A more comprehensive understanding of host–pathogen interaction can lead to new and improved strategies to maintain health. In particular, host genetic factors can lead to a more effective response to pathogens, hereafter termed resistance. Fayoumi and Leghorn chicken lines have demonstrated relative resistance and susceptibility, respectively, to the Newcastle disease virus (NDV) vaccine strain and many other pathogens. This biological model was used to better understand the host response to a vaccine strain of NDV across three tissues and time points, using RNA-seq. Analyzing the Harderian gland, trachea, and lung tissues together using weighted gene co-expression network analysis (WGCNA) identified important genes that were co-expressed and associated with parameters including: genetic line, days post-infection (dpi), challenge status, sex, and tissue. Pathways and driver genes, such as <em>EIF2AK2</em>, <em>MPEG1</em>, and <em>TNFSF13B</em>, associated with challenge status, dpi, and genetic line were of particular interest as candidates for disease resistance. Overall, by jointly analyzing the three tissues, this study identified genes and gene networks that led to a more comprehensive understanding of the whole animal response to lentogenic NDV than that obtained by analyzing the tissues individually.</p>
dc.description.comments <p>This article is published as Deist, Melissa S., Rodrigo A. Gallardo, Jack Dekkers, Huaijun Zhou, and Susan J. Lamont. "Novel combined tissue transcriptome analysis after lentogenic Newcastle disease virus challenge in inbred chicken lines of differential resistance." <em>Frontiers in Genetics</em> 11 (2020): 11. DOI: <a href="" target="_blank">10.3389/fgene.2020.00011</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1552
dc.identifier.contextkey 19353053
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/552
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 00:55:49 UTC 2022
dc.source.uri 10.3389/fgene.2020.00011
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords chickens
dc.subject.keywords RNA-seq
dc.subject.keywords weighted gene co-expression network analysis
dc.subject.keywords trachea
dc.subject.keywords lung
dc.subject.keywords Harderian gland
dc.title Novel Combined Tissue Transcriptome Analysis After Lentogenic Newcastle Disease Virus Challenge in Inbred Chicken Lines of Differential Resistance
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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