Effects of in Vitro and in Vivo Administration of Recombinant Bovine Interferon-)' on Bovine Neutrophil Responses to Brucella abortus

Thumbnail Image
Date
1989
Authors
Canning, Peter
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Person
Research Projects
Organizational Units
Organizational Unit
Veterinary Microbiology and Preventive Medicine
Our faculty promote the understanding of causes of infectious disease in animals and the mechanisms by which diseases develop at the organismal, cellular and molecular levels. Veterinary microbiology also includes research on the interaction of pathogenic and symbiotic microbes with their hosts and the host response to infection.
Journal Issue
Is Version Of
Versions
Series
Abstract

The effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-γ on in vitro bovine polymorphonuclear leukocyte functions and the survival of Brucella abortus were determined. Activation of neutrophils in vitro with interferon-γ resulted in enhanced production of O2 and myelopeoroxidase-H2O2-halide activity by neutrophils in the presence of B. abortus. The improved iodination responses were correlated with an enhanced ability to perform iodination in the presence of 5′-guanosine monophosphate and adenine which have previously been shown to contribute to inhibition of neutrophil myeloperoxidase-H2O2-halide activity by B. abortus. The ability of opsonized B. abortus to survive in the presence of neutrophils activated in vitro or in vivo was partially decreased by ∼10% of control when compared to survival rates within control phagocytes. These results suggest that activation of neutrophils with recombinant interferon-γ partially enhances their oxidative metabolic responses, resulting in a slightly enhanced ability to kill virulent B. abortus.

Comments

This article is from Veterinary Immunology and Immunopathology 20 (1989): 119, doi:10.1016/0165-2427(89)90093-7.

Description
Keywords
Citation
DOI
Copyright
Collections