Effects of in Vitro and in Vivo Administration of Recombinant Bovine Interferon-)' on Bovine Neutrophil Responses to Brucella abortus

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1989
Authors
Canning, Peter
Roth, James
Roth, James
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Roth, James
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Veterinary Microbiology and Preventive Medicine
Abstract

The effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-γ on in vitro bovine polymorphonuclear leukocyte functions and the survival of Brucella abortus were determined. Activation of neutrophils in vitro with interferon-γ resulted in enhanced production of O2 and myelopeoroxidase-H2O2-halide activity by neutrophils in the presence of B. abortus. The improved iodination responses were correlated with an enhanced ability to perform iodination in the presence of 5′-guanosine monophosphate and adenine which have previously been shown to contribute to inhibition of neutrophil myeloperoxidase-H2O2-halide activity by B. abortus. The ability of opsonized B. abortus to survive in the presence of neutrophils activated in vitro or in vivo was partially decreased by ∼10% of control when compared to survival rates within control phagocytes. These results suggest that activation of neutrophils with recombinant interferon-γ partially enhances their oxidative metabolic responses, resulting in a slightly enhanced ability to kill virulent B. abortus.

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This article is from Veterinary Immunology and Immunopathology 20 (1989): 119, doi:10.1016/0165-2427(89)90093-7.

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