Effects of in Vitro and in Vivo Administration of Recombinant Bovine Interferon-)' on Bovine Neutrophil Responses to Brucella abortus

dc.contributor.author Canning, Peter
dc.contributor.author Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T04:40:11.000
dc.date.accessioned 2020-07-07T05:15:16Z
dc.date.available 2020-07-07T05:15:16Z
dc.date.issued 1989
dc.description.abstract <p>The effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-γ on in vitro bovine polymorphonuclear leukocyte functions and the survival of <em>Brucella abortus</em> were determined. Activation of neutrophils in vitro with interferon-γ resulted in enhanced production of O<sub>2</sub><sup>−</sup> and myelopeoroxidase-H<sub>2</sub>O<sub>2</sub>-halide activity by neutrophils in the presence of <em>B. abortus</em>. The improved iodination responses were correlated with an enhanced ability to perform iodination in the presence of 5′-guanosine monophosphate and adenine which have previously been shown to contribute to inhibition of neutrophil myeloperoxidase-H<sub>2</sub>O<sub>2</sub>-halide activity by <em>B. abortus</em>. The ability of opsonized <em>B. abortus</em> to survive in the presence of neutrophils activated in vitro or in vivo was partially decreased by ∼10% of control when compared to survival rates within control phagocytes. These results suggest that activation of neutrophils with recombinant interferon-γ partially enhances their oxidative metabolic responses, resulting in a slightly enhanced ability to kill virulent <em>B. abortus</em>.</p>
dc.description.comments <p>This article is from <em>Veterinary Immunology and Immunopathology </em>20 (1989): 119, doi:<a href="http://dx.doi.org/10.1016/0165-2427(89)90093-7" id="x-ddDoi" target="_blank">10.1016/0165-2427(89)90093-7</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/55/
dc.identifier.articleid 1056
dc.identifier.contextkey 7786446
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/55
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92361
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/55/1989_Roth_EffectsInVitro.pdf|||Sat Jan 15 00:55:08 UTC 2022
dc.source.uri 10.1016/0165-2427(89)90093-7
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Effects of in Vitro and in Vivo Administration of Recombinant Bovine Interferon-)' on Bovine Neutrophil Responses to Brucella abortus
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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