Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans

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Peters, Stephanie
Pascoe, Ben
Wu, Zuowei
Bayliss, Sion C.
Zeng, Ximin
Edwinson, Adam
Veerabadhran-Gurunathan, Sakteesh
Jawahir, Selina
Calland, Jessica K.
Mourkas, Evangelos
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Springer Nature
Zhang, Qijing
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Veterinary Microbiology and Preventive Medicine
Our faculty promote the understanding of causes of infectious disease in animals and the mechanisms by which diseases develop at the organismal, cellular and molecular levels. Veterinary microbiology also includes research on the interaction of pathogenic and symbiotic microbes with their hosts and the host response to infection.
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Campylobacter enterocolitis may lead to post-infection irritable bowel syndrome (PI-IBS) and while some C. jejuni strains are more likely than others to cause human disease, genomic and virulence characteristics promoting PI-IBS development remain uncharacterized. We combined pangenome-wide association studies and phenotypic assays to compare C. jejuni isolates from patients who developed PI-IBS with those who did not. We show that variation in bacterial stress response (Cj0145_phoX), adhesion protein (Cj0628_CapA), and core biosynthetic pathway genes (biotin: Cj0308_bioD; purine: Cj0514_purQ; isoprenoid: Cj0894c_ispH) were associated with PI-IBS development. In vitro assays demonstrated greater adhesion, invasion, IL-8 and TNFα secretion on colonocytes with PI-IBS compared to PI-no-IBS strains. A risk-score for PI-IBS development was generated using 22 genomic markers, four of which were from Cj1631c, a putative heme oxidase gene linked to virulence. Our finding that specific Campylobacter genotypes confer greater in vitro virulence and increased risk of PI-IBS has potential to improve understanding of the complex host-pathogen interactions underlying this condition.
This article is published as Peters, Stephanie, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan et al. "Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans." Communications Biology 4, no. 1 (2021): 1015. DOI: 10.1038/s42003-021-02554-8. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.