Developing the CRISPR Interference System to Understand Bacterial Gene Function

dc.contributor.author Weems, Megan
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-14T10:01:28.000
dc.date.accessioned 2020-07-07T05:10:39Z
dc.date.available 2020-07-07T05:10:39Z
dc.date.issued 2014-04-15
dc.description.abstract <p>Gene function in bacteria is typically studied by isolation and characterization of mutants with loss of gene function. Since isolation or construction of specific mutants can be problematic when studying multiple genes with redundant function or bacteria with poorly developed genetic systems, we have developed an alternative genetic approach to study gene function. For this, we have exploited the observation that CRISPR (clustered regularly interspaced short palindromic repeats), which normally serves to protect bacterial cells against transfer of foreign DNA, has been modified as a gene silencing system. We have improved the use of a mutationally altered Cas9 protein [Qi, L], along with co-expression of an RNA guide molecule, to repress expression of a number of genes in E. coli K-12 strains. This system has been used to silence expression of selected genes. This research should lead to a better understanding of gene function, as well as a general method to study pathogenic bacteria in their natural hosts.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/52/
dc.identifier.articleid 1051
dc.identifier.contextkey 5914612
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2014/presentations/52
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91617
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/52/WeemsMegan.pdf|||Sat Jan 15 00:46:21 UTC 2022
dc.subject.disciplines Bacteriology
dc.title Developing the CRISPR Interference System to Understand Bacterial Gene Function
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Microbiology
File
Original bundle
Now showing 1 - 1 of 1
Name:
WeemsMegan.pdf
Size:
324.61 KB
Format:
Adobe Portable Document Format
Description: