Transcriptional Profiling of the Caloric Restriction in Key Metabolic Tissues of Pigs Differing in Feed Efficiency Lkhagvadorj, Sender Qu, Long Cai, Weiguo Couture, Oliver Anderson, Lloyd Dekkers, Jack Nettleton, Daniel Tuggler, Christopher 2018-08-25T20:57:05.000 2020-06-29T23:32:53Z 2020-06-29T23:32:53Z Fri Jan 01 00:00:00 UTC 2010 2010-01-01
dc.description.abstract <p>Residual feed intake is a measure of feed efficiency, where low RFI denotes high feed efficiency. Caloric restriction (CR) is associated with feed efficiency in livestock species and to human health benefits such as longevity and cancer prevention. We have developed pig lines that differ in RFI and we are interested in identifying the genes and pathways that underlie feed efficiency. Prepubertal Yorkshire gilts with low RFI (n=10) or high RFI (n=10) were fed ad libitum or at 80% of maintenance for 8 days. We measured serum metabolites and generated transcriptional profiles of liver and subcutaneous adipose tissue on these animals. Overall, 6,114 genes in fat and 305 genes in liver were differentially expressed (DE) in response to CR, and 311 genes in fat and 147 genes in liver were DE due to RFI differences. Pathway analyses of CR-induced DE genes indicated a dramatic switch to a conservation mode of energy usage by down-regulating lipogenesis and steroidogenesis in both liver and fat. Interestingly, CR altered expression of genes in immune and cell cycle/apoptotic pathways in fat, which may explain part of the CR-driven lifespan enhancement. In-silico analysis of transcription factors revealed ESR1 as a putative regulator of the adaptive response to CR, as several targets of ESR1 in our DE fat genes were annotated as cell cycle/apoptosis genes. The lipid metabolic pathway was overrepresented by down-regulated genes due to both CR and low RFI. We propose a common energy conservation mechanism, which may be controlled by PPARA, PPARG, and/or CREB in both CR and feed efficient pigs.</p>
dc.identifier archive/
dc.identifier.articleid 1597
dc.identifier.contextkey 3393879
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_air/vol656/iss1/79
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R2558
dc.source.bitstream archive/|||Sat Jan 15 01:55:23 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R2558
dc.title Transcriptional Profiling of the Caloric Restriction in Key Metabolic Tissues of Pigs Differing in Feed Efficiency
dc.type article
dc.type.genre swine
dspace.entity.type Publication
relation.isJournalIssueOfPublication d2f1e264-8d1c-46ff-bfdb-6c982aaaf7bd
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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