Detoxification of Corn Stover and Corn Starch Pyrolysis Liquors by Ligninolytic Enzymes of Phanerochaete chrysosporium

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2005-01-01
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Khiyami, Mohammad
Pometto, Anthony
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Phanerochaete chrysosporium (ATCC 24725) shake flask culture with 3 mM veratryl alcohol addition on day 3 was able to grow and detoxify different concentrations of diluted corn stover (Dcs) and diluted corn starch (Dst) pyrolysis liquors [10, 25, and 50% (v/v)] in defined media. GC-MS analysis of reaction products showed a decrease and change in some compounds. In addition, the total phenolic assay with Dcs samples demonstrated a decrease in the phenolic compounds. A bioassay employing Lactobacillus casei growth and lactic acid production was developed to confirm the removal of toxic compounds from 10 and 25% (v/v) Dcs and Dst by the lignolytic enzymes, but not from 50% (v/v) Dcs and Dst. The removal did not occur when sodium azide or cycloheximide was added to Ph. chrysosporium culture media, confirming the participation of lignolytic enzymes in the detoxification process. A concentrated enzyme preparation decreased the phenolic compounds in 10% (v/v) corn stover and corn starch pyrolysis liquors to the same extent as the fungal cultures.

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Reprinted (adapted) with permission from J. Agric. Food Chem., 2005, 53 (8), pp 2969–2977. Copyright 2005 American Chemical Society.

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Sat Jan 01 00:00:00 UTC 2005
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