Genetic typing and heat resistance of Escherichia coli from pig carcasses during slaughter in a UK slaughterhouse

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2009-01-01
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Wei, S.
Richards, P.
Dodd, Christine
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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During pork production carcasses are exposed to thennal treatments during the scald and singeing processes. In a study of cross contamination and survival of enteric contaminants during pork slaughter, changes in Escherichia coli and Salmonella population structures were analysed using ERIC-PCR following various stages in the process. Changes in genomic groups of E. coli isolates were identified following processes involving heat procedures. Selected strains from different genomic groups were tested for heat-resistance at 55°C. Differences in heat tolerance were found between isolates of different genotypes, and between exponential and stationary stages of growth of the same strain. The strains which survived the heating stages in the pork processing and which were more heat-resistant showed the induction of RpoS after 5h culture, but no significant RpoS-induction was observed in a heat-sensitive strain. This also correlated with the greater resistance seen in stationary phase of heat resistant strains. This work suggests processing may select subpopulations based on their greater heat resistances.

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Thu Jan 01 00:00:00 UTC 2009