PCR Typing of Tetracycline resistance Determinant (Tet A-E) in Salmonella sp.

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2001-01-01
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Pintérová, D.
Škardová, O.
Ružičková, H.
Demnerová, K.
Pazlarová, J.
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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The objectives of this study were the confirmation of Salmonella sp. presence in activated sludge of Prague waste water facility. Found serotypes were diverse, including more then 30 serotypes. In the collection of 90 strains the antibiotic (A TB) resistance was studied. The distribution of tetracycline resistance determinants Tet A-E was studied by PCR in 10 tetracycline resistant Salmonella sp. Tet resistance was more common in certain serotypes. All S. Hadar isolates were resistant to tetracycline yielding product responding to Tet A. Strain S. Virchow was found to bear Tet B determinant. In three phenotypically tetracycline resistant strains of S. Typhimurium PT DT I 04 were not found any tested determinants. To assign the location of found determinants tet(A) and tet(B) the digoxigenine labelled probes were prepared. Both chromosomal and plasmid DNA from tested strains was hybridized with these probes. Results indicated a high prevalence of the Tet A determinant.

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