Epimural Indicator Phylotypes of Transiently-Induced Subacute Ruminal Acidosis in Dairy Cattle

dc.contributor.author Esser, Stephan Schmitz
dc.contributor.author Wetzels, Stefanie
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.author Mann, Evelyne
dc.contributor.author Metzel-Zebeli, Barbara
dc.contributor.author Pourazad, Poulad
dc.contributor.author Qumar, Muhammad
dc.contributor.author Klevenhusen, Fenja
dc.contributor.author Pinior, Beate
dc.contributor.author Wagner, Martin
dc.contributor.author Zebeli, Qendrim
dc.contributor.department Animal Science
dc.date 2018-02-19T05:52:28.000
dc.date.accessioned 2020-06-29T23:40:04Z
dc.date.available 2020-06-29T23:40:04Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p>The impact of a long-term subacute rumen acidosis (SARA) on the bovine epimural bacterial microbiome (BEBM) and its consequences for rumen health is poorly understood. This study aimed to investigate shifts in the BEBM during a long-term transient SARA model consisting of two concentrate-diet-induced SARA challenges separated by a 1-week challenge break. Eight cows were fed forage and varying concentrate amounts throughout the experiment. In total, 32 rumen papilla biopsies were taken for DNA isolation (4 sampling time points per cow: at the baseline before concentrate was fed, after the first SARA challenge, after the challenge break, and after the second SARA challenge). Ruminal pH was continuously monitored. The microbiome was determined using Illumina MiSeq sequencing of the 16S rRNA gene (V345 region). In total 1,215,618 sequences were obtained and clustered into 6833 operational taxonomic units (OTUs). <em>Campylobacter</em> and <em>Kingella</em> were the most abundant OTUs (16.5 and 7.1%). According to ruminal pH dynamics, the second challenge was more severe than the first challenge. Species diversity estimates and evenness increased during the challenge break compared to all other sampling time points (<em>P</em> < 0.05). During both SARA challenges, <em>Kingella-</em> and <em>Azoarcus</em>-OTUs decreased (0.5 and 0.4 fold-change) and a dominant <em>Ruminobacter</em>-OTU increased during the challenge break (18.9 fold-change; <em>P</em> < 0.05). qPCR confirmed SARA-related shifts. During the challenge break noticeably more OTUs increased compared to other sampling time points. Our results show that the BEBM re-establishes the baseline conditions slower after a SARA challenge than ruminal pH. Key phylotypes that were reduced during both challenges may help to establish a bacterial fingerprint to facilitate understanding effects of SARA conditions on the BEBM and their consequences for the ruminant host.</p>
dc.description.comments <p>This article is published as Wetzels, Stefanie U., Evelyne Mann, Barbara U. Metzler-Zebeli, Poulad Pourazad, Muhammad Qumar, Fenja Klevenhusen, Beate Pinior, Martin Wagner, Qendrim Zebeli, and Stephan Schmitz-Esser. "Epimural indicator phylotypes of transiently-induced subacute ruminal acidosis in dairy cattle." Frontiers in microbiology 7 (2016). <a href="http://dx.doi.org/10.3389" target="_blank">10.3389/fmicb.2016.00274</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/343/
dc.identifier.articleid 1343
dc.identifier.contextkey 11201017
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/343
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9762
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/343/2016_Esser_EpimuralIndicator.pdf|||Fri Jan 14 23:42:00 UTC 2022
dc.source.uri 10.3389/fmicb.2016.00274
dc.subject.disciplines Agricultural Economics
dc.subject.disciplines Agriculture
dc.subject.disciplines Dairy Science
dc.subject.disciplines Meat Science
dc.subject.keywords Campylobacter
dc.subject.keywords Kingella
dc.subject.keywords rumen epithelium
dc.subject.keywords microbiota
dc.subject.keywords long-term subacute rumen acidosis
dc.subject.keywords cattle
dc.subject.keywords feeding
dc.subject.keywords 16S rRNA gene amplicon sequencing
dc.title Epimural Indicator Phylotypes of Transiently-Induced Subacute Ruminal Acidosis in Dairy Cattle
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 247d6138-be53-44be-9e88-220424e98124
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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