Comparison of DNA extraction methods to detect Salmonella spp. from pig faeces and pork

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2011-01-01
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Lettini, A.
Barco, Lisa
dalla Pozza, M.
Ramon, E.
Longo, A.
Marzia, Mancin
Ricci, Antonia
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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Abstract

The quality of DNA extract significantly influences the outcome of PCR-based detection methods. Performances of four different DNA extraction methods were evaluated for their ability to recover Salmonella DNA from artificially contaminated specimens. Swine faecal and pork samples were spiked with known concentration of Salmonella Typhimurium, DNA was then extracted by each of the methods considered and finally tested by a commercial Salmonella Real Time kit.

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Sat Jan 01 00:00:00 UTC 2011