Nanoparticle-assisted laser desorption/ionization mass spectrometry: novel sample preparation methods and nanoparticle screening for plant metabolite imaging

dc.contributor.advisor Young-Jin Lee
dc.contributor.author Yagnik, Gargey
dc.contributor.department Chemistry
dc.date 2018-08-11T13:00:47.000
dc.date.accessioned 2020-06-30T02:59:57Z
dc.date.available 2020-06-30T02:59:57Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.embargo 2001-01-01
dc.date.issued 2015-01-01
dc.description.abstract <p>The main goal of the presented research is development of nanoparticle based matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). This dissertation includes the application of previously developed data acquisition methods, development of novel sample preparation methods, application and comparison of novel nanoparticle matrices, and comparison of two nanoparticle matrix application methods for MALDI-MS and MALDI-MS imaging. Chapter 1 presents background information about MALDI-MS and a general workflow for MALDI-MS imaging experiments. Chapter 6 provides a summary of the presented work and possible future directions.</p> <p>Chapter 2 describes a “multiplex” MALDI-MS imaging technique applied to study latent human fingerprints. In this study, we applied previously developed multiplex data acquisition techniques for the detection of endogenous and exogenous compounds with structural information in a single MALDI-MS imaging experiment. This study also depicts the importance of the sublimation matrix application method for analysis of very delicate samples such as latent fingerprints.</p> <p>Chapter 3 presents the development of novel sample preparation methods for nanoparticle-assisted laser desorption/ionization-mass spectrometry -mass spectrometry (NALDI-MS) imaging of metabolites in plant tissues. In this chapter, we developed a novel fracturing method for MALDI-MS/ NALDI-MS imaging of rice leaf to study a plant-pathogen interactions. We successfully detected and imaged diterpenoids produced against the Xanthomonas oryzae pv. oryzae infection in rice leaves.</p> <p>Chapter 4 is focused on novel nanoparticle (NP) matrix development for NALDI-MS of low-molecular weight plant metabolites. This large-scale NP screening was inspired by the success of the metabolite detection/imaging in Chapter 3. In this work, we studied three different classes of NPs for desorption/ionization of a wide variety of plant metabolites and sought to shed some light on the NALDI mechanism.</p> <p>Chapter 5 is a further extension of the study in Chapter 4, in which we compare two NP application methods for efficiency of desorption/ionization of plant metabolites and homogeneity of application. We compare application of a nanoparticle suspension by oscillating capillary nebulizer and solvent-free sputter coating of metal NPs. The work presented in Chapters 4 and 5 will be helpful for development of novel matrices for analysis and imaging of low-molecular weight metabolites that are difficult to ionize using traditional MALDI-MS matrices.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/14896/
dc.identifier.articleid 5903
dc.identifier.contextkey 8436152
dc.identifier.doi https://doi.org/10.31274/etd-180810-4494
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/14896
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/29080
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/14896/Yagnik_iastate_0097E_15280.pdf|||Fri Jan 14 20:28:23 UTC 2022
dc.subject.disciplines Analytical Chemistry
dc.subject.keywords Analytical Chemistry
dc.title Nanoparticle-assisted laser desorption/ionization mass spectrometry: novel sample preparation methods and nanoparticle screening for plant metabolite imaging
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.discipline Analytical Chemistry
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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