Pilot study on the prevalence of salmonella in slaughter pigs in Germany: III. Detection of salmonellae by PCR, serovar distribution and population analysis of isolates

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1997
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Helmuth, R.
Käsbohrer, A.
Geue, L.
Rabsch, W.
Protz, D.
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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In a survey on the prevalence of Salmonellae in German slaughter pigs, almost 12,000 pig carcasses were investigated by standard cultural, immunological and PCR technique's. Seven abattoirs distributed all over Germany were included into the study. Each abattoir was investigated on ten weekly sampling occasions. In total 6% of all slaughter pigs were positive in faecal or lymphnode material. This number increased to I 0% when surface swabs were evaluated as well. From all sources nearly 1400 bacterial isolates were obtained and characterised by traditional and molecular typing methods. Typing at the National Reference Laboratory for Salmonella (NRL) follows a hierarchic protocol. At the beginning the serotype is determined by using poly- and monoclonal antisera for standard slide agglutination. In total 28 serotypes could be detected.

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Wed Jan 01 00:00:00 UTC 1997