Quick PCR to detect M. tuberculosis and M. bovis in swine blood samples

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2005-01-01
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Mainda, Geoffrey
Webao, Qi
Manlin, Luo
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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The disease control strategies in livestock are the key areas to enhance improvement of livestock production and safety of livestock product consumers. Therefore, reliable techniques for quick detection and specific identification of disease causing agents need to be developed. In this study a nested PCR was developed using primer primier 5.0 software for primer design targeting IS1081 gene conserved regions in both M. tuberculosis and M. bovis. Two sets of primers TB-Q1/TB-Q2 and TB-B1/TB-B2, were designed to detect M. tuberculosis and M. bovis DNA extracted from blood samples. The findings were, the assay is sensitive enough to detect up to 1.35fg of the antigen DNA with 100% specificity.

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Sat Jan 01 00:00:00 UTC 2005