Metabolic characteristics of the ketotic state in the bovine

Thumbnail Image
Mills, Scott
Major Professor
Committee Member
Journal Title
Journal ISSN
Volume Title
Research Projects
Organizational Units
Organizational Unit
Animal Science

The Department of Animal Science originally concerned itself with teaching the selection, breeding, feeding and care of livestock. Today it continues this study of the symbiotic relationship between animals and humans, with practical focuses on agribusiness, science, and animal management.

The Department of Animal Husbandry was established in 1898. The name of the department was changed to the Department of Animal Science in 1962. The Department of Poultry Science was merged into the department in 1971.

Historical Names

Journal Issue
Is Version Of

Gluconeogenic and ketogenic capacities of liver slices as well as metabolite concentrations in plasma and liver were examined in five dairy cows before and after an experimental lactation ketosis, and in four dairy steers treated with phlorizin (P) and/or 1,3-butanediol (BD). Liver biopsies were performed in lactating cows once before parturition and four times within 50 days following parturition. All cows were restricted approximately 15% from ad libitum feed consumption at 14 days postpartum. Four cows became ketotic (average 36 days postpartum) and were characterized during ketosis, compared to early lactation, by: a 66 (+OR-) 6% decrease in propionate, lactate, ALA, ASP, and GLU conversion to glucose; a 47 (+OR-) 6% decrease in the same substrates oxidized to carbon dioxide: and a 70% decrease in (beta)-hydroxybutyrate (BHBA) synthesis when incubated with butyrate. Also, ketotic cows lost body weight at a rate of 3.1 kg/day, and during ketosis, compared to prepartum, showed; a 10 to 15 fold increase in plasma and hepatic ketone bodies (KB), a 10 fold increase in hepatic triglyceride, and a 90% decrease in hepatic glycogen. Much less drastic changes were noted for the nonketotic cow;Liver biopsies were performed on the four steers at the end of each period of a 4 x 4 Latin square trial, and following 6 days of feed restriction in three of the four steers after completion of the Latin square trial. Treatments for the Latin square were: (C) is control, hay plus corn ration; (BD) is C + butanediol added to the ration; (P) is C + twice daily subcutaneous injections of 1 g of phlorizin; and (PBD) is C + P + BD. Glucose production (nmoles substrate converted to glucose/100 mg liver x 1 h) from propionate, lactate, and ASP when fed C was 974, 334, and 101, respectively. Feeding BD increased plasma and hepatic BHBA, and depressed glucose production from propionate (22%) and lactate (35%) compared to C. Phlorizin (P or PBD) or restricted feeding increased glucose production from propionate (32 to 74%), lactate (55 to 160%), and ASP (32-41%). KB (10 mM) plus 1 mM stearate added in vitro, completely negated the increased glucose production due to feed restriction. KB suppress hepatic metabolism, which may be an important contributor to ketosis development.

Fri Jan 01 00:00:00 UTC 1982