Occurrence and movement of total and tylosin-resistant enterococci, erm genes and tylosin in tile-drained agricultural fields receiving swine manure application

dc.contributor.advisor Michelle L. Soupir
dc.contributor.advisor Thomas B. Moorman
dc.contributor.author Garder, Jason
dc.contributor.department Civil, Construction, and Environmental Engineering
dc.date 2018-08-11T14:34:39.000
dc.date.accessioned 2020-06-30T02:46:11Z
dc.date.available 2020-06-30T02:46:11Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.embargo 2013-06-05
dc.date.issued 2012-01-01
dc.description.abstract <p>The use of tylosin at subtherapeutic levels by the swine industry provides selective pressure for the development of antibiotic resistance in gastrointestinal bacteria. The land application of swine manure to drained agricultural fields might introduce elevated levels of total and tylosin-resistant enterococci, erm genes and tylosin. The goal of this study was to develop an understanding of the occurrence and transport of total and tylosin-resistant enterococci, erm genes and tylosin in tile-drained chisel plow and no-till agricultural fields that have received multi-year application of liquid swine manure through injection over two growing seasons.</p> <p>Resistance to tylosin in manure, soil and water samples was investigated at the field scale level using phenotypic based (membrane filtration) and genotypic based (qPCR) methods and compared with samples from control plots treated with urea and ammonium nitrate (UAN). Tylosin was quantified using LC-MS/MS. Plots in a corn-soybean rotation were identified for sampling from 2010-2012. Soil samples were collected from each manure plot, from both the direct area of injection and from the area between the manure bands and from control plots. Each one-acre plot is drained separately and tile water samples were collected directly from the discharge tile line weekly while the tiles were flowing. The results of this study suggest that tylosin usage has increased the short-term occurrence of total and tylosin-resistant enterococci, erm genes, and tylosin in soils, but has had minimal effect on tile drainage water quality under dryer than average conditions</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/12933/
dc.identifier.articleid 3940
dc.identifier.contextkey 4188261
dc.identifier.doi https://doi.org/10.31274/etd-180810-2584
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/12933
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/27122
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/12933/Garder_iastate_0097M_13087.pdf|||Fri Jan 14 19:33:40 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Environmental Engineering
dc.subject.disciplines Water Resource Management
dc.title Occurrence and movement of total and tylosin-resistant enterococci, erm genes and tylosin in tile-drained agricultural fields receiving swine manure application
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
thesis.degree.level thesis
thesis.degree.name Master of Science
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