Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium Fakhr, Mohamed Nolan, Lisa Logue, Catherine
dc.contributor.department Veterinary Microbiology and Preventive Medicine 2018-02-13T10:37:20.000 2020-07-07T05:14:50Z 2020-07-07T05:14:50Z Sat Jan 01 00:00:00 UTC 2005 2013-05-06 2005-05-01
dc.description.abstract <p>Nontyphoidal salmonellae are among the leading causes of food-borne disease in the United States. Because of the importance of <em>Salmonella enterica</em> in food-borne disease, numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the “gold standard” technique in typing <em>Salmonella</em>. The aim of this study was to compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing <em>Salmonella enterica</em> serovar Typhimurium clinical isolates. A total of 85 <em>Salmonella</em> Typhimurium clinical isolates from cattle were used in this study. PFGE using XbaI was performed on the 85 isolates by the Centers for Disease Control and Prevention method, and data were analyzed using the BioNumerics software package. Fifty PFGE profiles were observed among the isolates, and these grouped into three major clusters. For the MLST analysis, the <em>manB</em>, <em>pduF</em>, <em>glnA</em>, and <em>spaM</em> genes were amplified by PCR from the same 85 isolates. DNA sequencing of these four genes, <em>manB</em>, <em>pduF</em>, <em>glnA</em>, and<em>spaM</em>, showed no genetic diversity among the isolates tested, with a 100% identity in nucleotide sequence. Moreover, the DNA sequences of the aforementioned genes showed 100% identity to the sequence reported in GenBank for the <em>S. enterica</em> serovar Typhimurium LT2 strain. Therefore, MLST, using these genes, lacks the discriminatory power of PFGE for typing <em>Salmonella enterica</em>serovar Typhimurium.</p>
dc.description.comments <p>This article is from <em>Journal of Clinical Microbiology</em> 43, no. 5 (May 2005): 2215–2219, doi:<a href="" target="_blank">10.1128/JCM.43.5.2215-2219.2005</a>.</p>
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dc.identifier.articleid 1014
dc.identifier.contextkey 4110034
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dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:32:29 UTC 2022
dc.source.uri 10.1128/​JCM.43.5.2215-2219.2005
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium
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dc.type.genre article
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relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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