Signaling dynamics in rice expressing constitutively active Nicotiana Protein Kinase 1 (NPK1)

dc.contributor.advisor Kan Wang Scarpa, Andréa
dc.contributor.department Theses & dissertations (Interdisciplinary) 2018-08-23T15:05:17.000 2020-06-30T07:38:23Z 2020-06-30T07:38:23Z Mon Jan 01 00:00:00 UTC 2007 2007-01-01
dc.description.abstract <p>Signaling components such as mitogen-activated protein kinases (MAPKs) regulate stress-induced pathways and are potentially a powerful means of genetically engineering plant tolerance to abiotic stress. The kinase domain of tobacco mitogen-activated protein kinase Nicotiana protein kinase 1 (NPK1) into rice cultivar Nipponbare. Four-week old transgenic and wild type plants treated with moderate (150mM) or severe (300mM) salt stress did not differ in visible signs of leaf damage, electrolyte leakage, malondialdehyde content or ion content within leaves. Expression of caNPK1 alters gene transcript abundance in the absence of stress as assessed by microarray analysis. Genes potentially related to flowering and development are downregulated while biotin biosynthesis and methionine recycling pathway as well as thirteen transcripts with matches to stress-related genes appear upregulated in the transgenic plants. Quantitative real-time PCR analysis indicates no significant difference in expression of several known rice MAPKs between caNPK1 transgenics and wild type.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 15660
dc.identifier.contextkey 6997480
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/14661
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 20:24:14 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Biology
dc.subject.keywords Agronomy;Plant physiology
dc.title Signaling dynamics in rice expressing constitutively active Nicotiana Protein Kinase 1 (NPK1)
dc.type article
dc.type.genre thesis
dspace.entity.type Publication Plant Physiology thesis Master of Science
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