Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

dc.contributor.author Cai, Weili
dc.contributor.author Johansen, Kristen
dc.contributor.author Jin, Ye
dc.contributor.author Girton, Jack
dc.contributor.author Johansen, Jorgen
dc.contributor.author Johansen, Kristen
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2019-08-11T17:42:45.000
dc.date.accessioned 2020-06-29T23:46:45Z
dc.date.available 2020-06-29T23:46:45Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.issued 2010-02-01
dc.description.abstract <p>Drosophila has long been a favorite model system for studying the relationship between chromatin structure and gene regulation due to the cytological advantages provided by the giant salivary gland polytene chromosomes of third instar larvae. In this tissue the chromosomes undergo many rounds of replication in the absence of cell division giving rise to approximately 1000 copies. The DNA remains aligned after each replicative cycle resulting in greatly enlarged chromosomes that provide a unique opportunity to correlate chromatin morphology with the localization of specific proteins. Consequently, there has been a high level of interest in defining the epigenetic modifications present at different genes and at different stages of the transcription process. An important tool for such studies is the labeling of polytene chromosomes with antibodies to the enzyme, transcription factor, or histone modification of interest. This video protocol illustrates the squash technique used in the Johansen laboratory to prepare Drosophila polytene chromosomes for antibody labeling.</p>
dc.description.comments <p>This article is published as Cai, W., Jin, Y., Girton, J., Johansen, J., Johansen, K.M. Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling. J. Vis. Exp. (36), e1748, doi:<a href="http://dx.doi.org/10.3791/1748" target="_blank">10.3791/1748</a> (2010). Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/248/
dc.identifier.articleid 1258
dc.identifier.contextkey 14712435
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/248
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10719
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/248/0-Jove_author_license.pdf|||Fri Jan 14 22:54:50 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/248/2010_Johansen_PreparationDrosophila.pdf|||Fri Jan 14 22:54:51 UTC 2022
dc.source.uri 10.3791/1748
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Entomology
dc.subject.disciplines Genetics
dc.subject.disciplines Molecular Biology
dc.subject.keywords Cellular Biology
dc.subject.keywords Issue 36
dc.subject.keywords polytene squash preparations
dc.subject.keywords antibody labeling
dc.subject.keywords chromosomes
dc.subject.keywords Drosophila
dc.title Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3829d759-f8ec-4502-a63a-1655d3b70ef5
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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