A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis

dc.contributor.author Yao, Changfu
dc.contributor.author Johansen, Kristen
dc.contributor.author Rath, Uttama
dc.contributor.author Maiato, Helder
dc.contributor.author Sharp, David
dc.contributor.author Girton, Jack
dc.contributor.author Johansen, Kristen
dc.contributor.author Johansen, Jorgen
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2019-09-22T08:46:36.000
dc.date.accessioned 2020-06-29T23:46:43Z
dc.date.available 2020-06-29T23:46:43Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.issued 2012-09-15
dc.description.abstract <p>The concept of a spindle matrix has long been proposed. Whether such a structure exists, however, and what its molecular and structural composition are have remained controversial. In this study, using a live-imaging approach in <em>Drosophila</em>syncytial embryos, we demonstrate that nuclear proteins reorganize during mitosis to form a highly dynamic, viscous spindle matrix that embeds the microtubule spindle apparatus, stretching from pole to pole. We show that this “internal” matrix is a distinct structure from the microtubule spindle and from a lamin B–containing spindle envelope. By injection of 2000-kDa dextran, we show that the disassembling nuclear envelope does not present a diffusion barrier. Furthermore, when microtubules are depolymerized with colchicine just before metaphase the spindle matrix contracts and coalesces around the chromosomes, suggesting that microtubules act as “struts” stretching the spindle matrix. In addition, we demonstrate that the spindle matrix protein Megator requires its coiled-coil amino-terminal domain for spindle matrix localization, suggesting that specific interactions between spindle matrix molecules are necessary for them to form a complex confined to the spindle region. The demonstration of an embedding spindle matrix lays the groundwork for a more complete understanding of microtubule dynamics and of the viscoelastic properties of the spindle during cell division.</p>
dc.description.comments <p>This article is published as Yao, Changfu, Uttama Rath, Helder Maiato, David Sharp, Jack Girton, Kristen M. Johansen, and Jørgen Johansen. "A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis." <em>Molecular biology of the cell</em>23, no. 18 (2012): 3532-3541. doi: <a href="https://doi.org/10.1091/mbc.e12-06-0429">10.1091/mbc.e12-06-0429</a>. </p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/241/
dc.identifier.articleid 1254
dc.identifier.contextkey 14683366
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/241
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10712
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/241/2012_Johansen_NuclearDerived.pdf|||Fri Jan 14 22:52:46 UTC 2022
dc.source.uri 10.1091/mbc.e12-06-0429
dc.subject.disciplines Biochemistry
dc.subject.disciplines Biophysics
dc.subject.disciplines Entomology
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Structural Biology
dc.title A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3829d759-f8ec-4502-a63a-1655d3b70ef5
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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