Using Resistant Prey Demonstrates That Bt Plants Producing Cry1Ac, Cry2Ab, and Cry1F Have No Negative Effects on Geocoris punctipes and Orius insidiosus

dc.contributor.author Tian, Jun-Ce
dc.contributor.author Hellmich, Richard
dc.contributor.author Long, Li-Ping
dc.contributor.author Wang, Xiang-Ping
dc.contributor.author Naranjo, Steven
dc.contributor.author Romeis, Jörg
dc.contributor.author Hellmich, Richard
dc.contributor.author Wang, Ping
dc.contributor.author Shelton, Anthony
dc.contributor.department Entomology
dc.date 2018-02-14T02:15:27.000
dc.date.accessioned 2020-06-30T02:25:03Z
dc.date.available 2020-06-30T02:25:03Z
dc.date.embargo 2014-07-03
dc.date.issued 2014-01-01
dc.description.abstract <p><em>Geocoris punctipes</em> (Say) and <em>Orius insidiosus</em> (Say) are generalist predators found in a wide range of crops, including cotton (<em>Gossypium hirsutum</em> L. ) and maize (<em>Zea mays</em> L. ), where they provide important biological control services by feeding on an array of pests, including eggs and small larvae of caterpillars. A high percentage of cotton and maize in the United States and several other countries are transgenic cultivars that produce one or more of the insecticidal Cry proteins of<em>Bacillus thuringiensis</em> Berliner (Bt). Here we quantify effects of three Cry proteins on the life history of these predators over two generations when they are exposed to these Cry proteins indirectly through their prey. To eliminate the confounding prey quality effects that can be introduced by Bt-susceptible prey, we used Cry1Ac/Cry2Ab-resistant <em>Trichoplusia ni</em> (Hübner) and Cry1 F-resistant<em>Spodoptera frugiperda</em> (J.E. Smith) in a series of tri-trophic studies. Survival, development, adult mass, fecundity, and fertility were similar when predators consumed larvae feeding on Cry1Ac/Cry2Ab cotton or Cry1 F maize compared with prey feeding on isogenic or near-isogenic cotton or maize. Repeated exposur of the same initial cohort over a second generation also resulted in no differences in life-history traits when feeding on non-Bt- or Bt-fed prey. Enzyme-linked immunosorbent assay showed that predators were exposed to Bt Cry proteins from their prey and that these proteins became increasingly diluted as they moved up the food chain. Results show a clear lack of effect of three common and widespread Cry proteins on these two important predator species. The use of resistant insects to eliminate prey quality effects provides a robust and meaningful assessment of exposure and hazard.</p>
dc.description.comments <p>This article is from <em>Environmental Entomology</em> 43 (2014): 242–251, doi:<a href="http://dx.doi.org/10.1603/EN13184">10.1603/EN13184</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/54/
dc.identifier.articleid 1053
dc.identifier.contextkey 5750668
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/54
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24176
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/54/2014_TianJC_UsingResistantPrey.pdf|||Sat Jan 15 00:52:42 UTC 2022
dc.source.uri 10.1603/EN13184
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Entomology
dc.subject.disciplines Genetics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Transgenic Bt crop
dc.subject.keywords Trichoplusia ni
dc.subject.keywords Spodoptera frugiperda
dc.subject.keywords Biological control service
dc.subject.keywords Prey quality
dc.title Using Resistant Prey Demonstrates That Bt Plants Producing Cry1Ac, Cry2Ab, and Cry1F Have No Negative Effects on Geocoris punctipes and Orius insidiosus
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3cca44d8-e1df-437d-a95a-d8e38963d2c1
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
File
Original bundle
Now showing 1 - 1 of 1
Name:
2014_TianJC_UsingResistantPrey.pdf
Size:
120.96 KB
Format:
Adobe Portable Document Format
Description:
Collections