After the recognition of hospital-associated MRSA and community-associated MRSA, recently a new lineage of MRSA was found in livestock, mainly in pigs (LA-MRSA). It was hypothesized that the presence of different livestock species could contribute to a higher prevalence of LA-MRSA on a farm.The aim of the study was to gain insights into the prevalence on Belgian mixed farms and to compare the antibacterial resistance pattern of MRSA.