Enantiomeric separations and microorganism studies with analytical separation techniques

dc.contributor.advisor Daniel W. Armstrong
dc.contributor.advisor Jacob W. Petrich
dc.contributor.author Bao, Ye
dc.contributor.department Chemistry
dc.date 2018-08-11T11:42:09.000
dc.date.accessioned 2020-06-30T02:33:38Z
dc.date.available 2020-06-30T02:33:38Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.embargo 2013-06-05
dc.date.issued 2008-01-01
dc.description.abstract <p>This dissertation is divided into two parts: Part I involves enantiomeric separations by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE); Part II describes a series of microorganism studies using CE.</p> <p>In Part I, Chapter 1 gives an overview of enantiomeric separations using HPLC and CE. Chapter 2 presents enantiomeric separations on a new synthetic LC chiral stationary phase (CSP), i.e., a pentaproline-based CSP. The enantiomeric separation ability of this new CSP was evaluated by injecting 194 racemates on the LC column. The chiral recognition mechanism was discussed and sample loading was briefly tested. Chapter 3 shows the enantiomeric separations of three groups of synthetic chiral compounds using CE: furan derivatives, fused polycycles and isochromene derivatives. Cyclodextrin-modified micellar capillary electrophoresis (CD-MCE) was utilized in this study. The reason for using this complicated method rather than the simplest capillary zone electrophoresis (CZE) was discussed and different types of cyclodextrin selectors were compared.</p> <p>In Part II, Chapter 4 serves as an introduction to this part of the dissertation: a review of work mainly from our group on microorganism studies using CE, focusing on the detection of microbial contamination and further evaluating the possibility of using CE to replace the traditional sterility test outlined in the U.S.Pharmacopeia. Chapter 5 presents a successful single-cell detection approach using a modification of a previously established procedure from our group, and thereby greatly widens the practicality and effectiveness of the method. Chapter 6 is a CE study on the use of ionic liquids (ILs) in the detection of microbial contamination. By using dicationic ILs as auxiliary buffer additives, we are able to further reduce the possibility of lysing cells by surfactants (such as CTAB).</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/11148/
dc.identifier.articleid 2227
dc.identifier.contextkey 2807425
dc.identifier.doi https://doi.org/10.31274/etd-180810-968
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/11148
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/25354
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/11148/Bao_iastate_0097E_10114.pdf|||Fri Jan 14 18:43:35 UTC 2022
dc.subject.disciplines Chemistry
dc.subject.keywords CE
dc.subject.keywords Chiral
dc.subject.keywords HPLC
dc.subject.keywords Microorganism
dc.subject.keywords Separation
dc.subject.keywords Sterility
dc.title Enantiomeric separations and microorganism studies with analytical separation techniques
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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