Genetic differentiation of some Glossina morsitans morsitans populations

dc.contributor.author Wohlford, D. L.
dc.contributor.author Krafsur, Elliot
dc.contributor.author Krafsur, E. S.
dc.contributor.author Griffiths, N. T.
dc.contributor.author Marquez, J. G.
dc.contributor.author Baker, M. D.
dc.contributor.department Entomology
dc.date 2019-09-21T10:07:46.000
dc.date.accessioned 2020-06-30T02:24:55Z
dc.date.available 2020-06-30T02:24:55Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 1999
dc.date.issued 1999-11-01
dc.description.abstract <p>To study the population structure of <em>Glossina morsitans morsitans</em> Westwood (Diptera: Glossinidae), polymerase chain reaction (PCR) and single‐strand conformational polymorphism (SSCP) methods were used to estimate mitochondrial DNA diversity at four loci in six natural populations from Zambia, Zimbabwe and Mozambique, and in two laboratory cultures. The Zambian and Zimbabwean samples were from a single fly belt. Four alleles were recorded at <em>12S and 16S1</em>, and five alleles at <em>16S2</em> and <em>COI</em>. Nucleotide sequencing confirmed their singularities. Chi‐square contingency tests showed that allele frequencies differed significantly among populations. Mean allele diversities in populations averaged over loci varied from 0.14 to 0.61. Little loss in haplotype diversity was detected in the laboratory cultures thereby indicating little inbreeding. Wright’s fixation index <em>F</em>ST in the natural populations was 0.088 ± 0.016, the correlation of haplotypes within populations relative to correlations in the total. A function of its inverse allows an estimate of the mean equivalent number of females exchanged per population per generation, 5.2. No correlation was detected between pairwise genetic distance measures and geographical distances. Drift explains the high degree of differentiation.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Wohlford, D. L., E. S. Krafsur, N. T. Griffiths, J. G. Marquez, and M. D. Baker. "Genetic differentiation of some Glossina morsitans morsitans populations." <em>Medical and veterinary entomology</em> 13, no. 4 (1999): 377-385, which has been published in final form at doi: <a href="https://doi.org/10.1046/j.1365-2915.1999.00185.x">10.1046/j.1365-2915.1999.00185.x</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/523/
dc.identifier.articleid 1543
dc.identifier.contextkey 15083915
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/523
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24158
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/523/1999_Krafsur_GeneticDifferentiationManuscript.pdf|||Sat Jan 15 00:48:19 UTC 2022
dc.source.uri 10.1046/j.1365-2915.1999.00185.x
dc.subject.disciplines Ecology and Evolutionary Biology
dc.subject.disciplines Entomology
dc.subject.disciplines Genetics
dc.subject.disciplines Population Biology
dc.subject.keywords Glossina morsitans
dc.subject.keywords mitochondrial variation
dc.subject.keywords population genetics
dc.subject.keywords tsetse flies
dc.title Genetic differentiation of some Glossina morsitans morsitans populations
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 85d1fdbf-0f97-46bb-a01d-9f769db1b921
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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