Observing Biosynthesis of Lignocellulosic Biomass in situ

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dc.contributor.author Fauser, Katrina
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2020-09-18T15:16:12.000
dc.date.accessioned 2021-02-26T00:34:30Z
dc.date.available 2021-02-26T00:34:30Z
dc.date.issued 2019-01-01
dc.description.abstract <p>The pollen grains of Arabidopsis thaliana produce a fast-growing pollen tube. The cell wall of this pollen tube can model lignocellulosic biomass. This project utilized a fucose analog to label the distribution of fucose in pollen tube cell wall during biosynthesis. Pollen grains were germinated on medium containing this alkyne-modified fucose analog, called FucAl. This allowed pollen grains to incorporate FucAl, rather than fucose, into cell walls. Following tube growth, cell walls containing FucAl were exposed to Alexa 594 and Alexa 647 Azides in a copper-catalyzed “click” reaction. This reaction between the azide and alkyne yields fluorescent product at the FucAl molecule’s location. This allowed us to localize where fucose belongs in the cell wall. These results confirm the success of the click reaction and suggest that fucose may be taken up by the pollen grain during tube elongation. Further studies are needed to shed light on the critical steps of this process. Once the methodology is optimized, it will be expanded to include biomass degradation. This simple biomass model was suitable for effective mapping of polysaccharide distribution during growth, and this project utilized correlative optical and fluorescence microscopy to study fucose delivery and utilization during cell wall biosynthesis.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/honors_posters/201904/projects/76/
dc.identifier.articleid 1254
dc.identifier.contextkey 19346587
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath honors_posters/201904/projects/76
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/96456
dc.relation.ispartofseries Honors Projects and Posters
dc.source.bitstream archive/lib.dr.iastate.edu/honors_posters/201904/projects/76/Fauser__Katrina.pdf|||Sat Jan 15 01:51:13 UTC 2022
dc.title Observing Biosynthesis of Lignocellulosic Biomass in situ
dc.type event
dc.type.genre event
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isSeriesOfPublication 78a1cb49-0dee-4c38-97a8-c1fd0b7a74ea
thesis.degree.discipline Genetics
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