Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene

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dc.contributor.author Konobe, Milly
dc.contributor.author Rodermel, Steven
dc.contributor.author Bailey, Theodore
dc.contributor.author Scott, M. Paul
dc.contributor.author Scott, Marvin
dc.contributor.department Agronomy
dc.contributor.department Statistics
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T14:47:25.000
dc.date.accessioned 2020-06-29T23:02:58Z
dc.date.available 2020-06-29T23:02:58Z
dc.date.issued 2013-06-14
dc.description.abstract <p>Transgenic agricultural crops with increased nutritive value present prospects for contributing to public health. However, their acceptance is poor in many countries due to the perception that genetic modification may cause unintended effects on expression of native genes in the host plant. Here, we tested effects of soybean ferritin transgene (SoyFer1, M64337) on transcript and protein levels of endogenous genes in maize. Results showed that the transgene was successfully introduced and expressed in the maize seed endosperm. mRNA abundance of seven tested iron homeostasis genes and seed storage protein genes differed significantly between seed samples positive and negative for the transgene. The PCR negative samples had higher zein and total protein content compared to the positive samples. However, PCR positive samples had significantly higher concentrations of calcium, magnesium, and iron. We have shown that the soybean ferritin transgene affected the expression of native iron homeostasis genes in the maize plant. These results underscore the importance of taking a holistic approach to the evaluation of transgenic events in target plants, comparing the transgenic plant to the untransformed controls.</p>
dc.description.comments <p>This article is published as Kanobe, Milly N., Steven R. Rodermel, Theodore Bailey, and Paul Scott. "Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene." <em>Frontiers in Plant Science</em> 4 (2013): 196, doi: <a href="https://doi.org/10.3389/fpls.2013.00196">10.3389/fpls.2013.00196</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/153/
dc.identifier.articleid 1150
dc.identifier.contextkey 10424461
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/153
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4481
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/153/2013_Scott_ChangesEndogenous.pdf|||Fri Jan 14 20:38:58 UTC 2022
dc.source.uri 10.3389/fpls.2013.00196
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Statistical Methodology
dc.subject.keywords soybean ferritin
dc.subject.keywords transgenic maize
dc.subject.keywords endogenous genes
dc.subject.keywords gene expression
dc.subject.keywords transcription
dc.subject.keywords iron
dc.title Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene
dc.type article
dc.type.genre article
dspace.entity.type Publication
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