Blufensin1 Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

dc.contributor.author Meng, Yan
dc.contributor.author Moscou, Matthew
dc.contributor.author Wise, Roger
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Bioinformatics and Computational Biology
dc.date 2018-02-17T08:43:12.000
dc.date.accessioned 2020-06-30T06:23:50Z
dc.date.available 2020-06-30T06:23:50Z
dc.date.issued 2009-01-01
dc.description.abstract <p>Plants have evolved complex regulatory mechanisms to control the defense response against microbial attack. Both temporal and spatial gene expression are tightly regulated in response to pathogen ingress, modulating both positive and negative control of defense. BLUFENSIN1 (BLN1), a small peptide belonging to a novel family of proteins in barley (<em>Hordeum vulgare</em>), is highly induced by attack from the obligate biotrophic fungus <em>Blumeria graminis</em> f. sp. <em>hordei</em> (<em>Bgh</em>), casual agent of powdery mildew disease. Computational interrogation of the <em>Bln1</em> gene family determined that members reside solely in the BEP clade of the Poaceae family, specifically, barley, rice (<em>Oryza sativa</em>), and wheat (<em>Triticum aestivum</em>). Barley stripe mosaic virus-induced gene silencing of <em>Bln1</em> enhanced plant resistance in compatible interactions, regardless of the presence or absence of functional <em>Mla</em> coiled-coil, nucleotide-binding site, Leu-rich repeat alleles, indicating that BLN1 can function in an <em>R</em>-gene-independent manner. Likewise, transient overexpression of <em>Bln1</em> significantly increased accessibility toward virulent <em>Bgh</em>. Moreover, silencing in plants harboring the <em>Mlo</em> susceptibility factor decreased accessibility to <em>Bgh</em>, suggesting that BLN1 functions in parallel with or upstream of MLO to modulate penetration resistance. Collectively, these data suggest that the grass-specific <em>Bln1</em> negatively impacts basal defense against <em>Bgh</em>.</p>
dc.description.comments <p>This article is from <em>Plant Physiology</em> 149 (2009): 271–285, doi:<a href="http://dx.doi.org/10.1104/pp.108.129031" target="_blank">10.1104/pp.108.129031</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/70/
dc.identifier.articleid 1068
dc.identifier.contextkey 7946606
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/70
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57786
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/70/2009_Meng_Blufensin1Negatively.pdf|||Sat Jan 15 01:40:19 UTC 2022
dc.source.uri 10.​1104/​pp.​108.​129031
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Plant Pathology
dc.title Blufensin1 Negatively Impacts Basal Defense in Response to Barley Powdery Mildew
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
File
Original bundle
Now showing 1 - 1 of 1
Name:
2009_Meng_Blufensin1Negatively.pdf
Size:
1.1 MB
Format:
Adobe Portable Document Format
Description:
Collections