Research Notes : United States : Cultivar identification by isozyme analysis

Date
1987-04-01
Authors
Doong, J.
Kiang, Y.
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Abstract

The purpose of this survey is to build a multiple enzyme system which may provide many biochemical characters to fingerprint cultivars. A total of 751 lines and cultivars of Glycine max have been screened for their enzyme zymogram types by using the technique of slab-gel electrophoresis (see Chiang, 1985; Doong, 1986; Gorman, 1983; Gorman and Kiang, 1977, 1978; Kiang and Gorman, 1983, for method). For 367 named cultivars, the zymogram types of eight enzymes and one seed protein (Ti) are listed in Table 1. Except for several cultivars, two of the eight enzymes, ADH and LAP, listed in Table 1 were mainly classified by Gorman (1983).

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