DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression

Date
2014-03-01
Authors
Nolan, Lisa
Xu, Haiping
Zhu, Xuenong
Hu, Yongsheng
Lamont, Susan
Li, Zhenhui
Zhang, Xiquan
Nie, Qinghua
Nolan, Lisa
Lamont, Susan
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Animal Science
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Animal Science
Abstract

Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively.IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.

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This article is from Scientific Reports 4 (2014): 4299, doi:10.1038/srep04299. Posted with permission.

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