DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression

dc.contributor.author Nolan, Lisa
dc.contributor.author Xu, Haiping
dc.contributor.author Zhu, Xuenong
dc.contributor.author Hu, Yongsheng
dc.contributor.author Lamont, Susan
dc.contributor.author Li, Zhenhui
dc.contributor.author Zhang, Xiquan
dc.contributor.author Nie, Qinghua
dc.contributor.author Nolan, Lisa
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2018-02-17T05:56:16.000
dc.date.accessioned 2020-06-29T23:38:49Z
dc.date.available 2020-06-29T23:38:49Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-03-01
dc.description.abstract <p>Avian pathogenic <em>Escherichia coli</em> (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively.<em>IL8</em>, <em>IL2RB</em>, and <em>IL1RAPL1</em> were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.</p>
dc.description.comments <p>This article is from <em>Scientific Reports</em> 4 (2014): 4299, doi:<a href="http://dx.doi.org/10.1038/srep04299" target="_blank">10.1038/srep04299</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/184/
dc.identifier.articleid 1182
dc.identifier.contextkey 7842202
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/184
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9587
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/184/2014_Lamont_DNAMethylome.pdf|||Fri Jan 14 21:41:22 UTC 2022
dc.source.uri 10.1038/srep04299
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords DNA methylation
dc.subject.keywords epigenomics
dc.title DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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