Investigation of the anti-virC2 transcript in Agrobacterium tumefaciens

dc.contributor.advisor Kan Wang Johnson, Abbagail
dc.contributor.department Agronomy 2018-08-11T17:46:42.000 2020-06-30T03:07:35Z 2020-06-30T03:07:35Z Fri Jan 01 00:00:00 UTC 2016 2001-01-01 2016-01-01
dc.description.abstract <p>Agrobacterium tumefaciens has long been an important tool for plant genetic transformation. Many important crop plants such as maize, soybean, wheat, and rice, as well as numerous other dicot plant species are able to be transformed using A. tumefaciens. While many potential regulatory RNA have been previously identified in A. tumefaciens, very few have been verified with a functional investigation. With the growing understanding of regulatory RNA, and its importance in gene regulation, it is essential to further classify regulatory RNA in the transformative tool A. tumefaciens. It is possible that regulatory RNA could be involved in virulence and their expression altered to increase transformation efficiency.</p> <p>In this work, a particular candidate regulatory RNA, anti-virC2, was selected and investigated. While anti-virC2 was identified previous to this research, the function of anti-virC2 has never before been examined. The location of anti-virC2 is on the Ti-plasmid antisense to the virulence gene virC2 and it was initially hypothesized that anti-virC2 acted to regulate the virC2 gene in cis.</p> <p>Through deletion of the promoter region, expression of the anti-virC2 transcript was reduced by ~39% in mutant strain C58 ∆Pavc2. Using this strain, changes in virC2 transcript and protein abundance were able to be observed and compared to wild type. In addition, other possible changes in virulence due to the drop in anti-virC2 expression were examined through Kalanchoe daigremontiana tumorigenesis assays and Arabidopsis thaliana infection experiments.</p> <p>This work shows that anti-virC2 does not act to regulate the cis encoded virC2 gene but may have some impact on virulence. Additional anti-virC2 targets were also predicted and may be investigated in future studies. As well as the findings made regarding anti-virC2, this study provides an example for the functional study of additional regulatory RNA and may serve as a model for the investigation of other potential regulatory RNAs.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 6950
dc.identifier.contextkey 11169373
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/15943
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 20:48:58 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Biology
dc.subject.disciplines Botany
dc.subject.keywords Agrobacterium tumefaciens
dc.subject.keywords regulatory RNA
dc.subject.keywords sRNA
dc.title Investigation of the anti-virC2 transcript in Agrobacterium tumefaciens
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a Plant Biology thesis Master of Science
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