Utilization of Reduced Haploid Vigor for Phenomic Discrimination of Haploid and Diploid Maize Seedlings

dc.contributor.author Ganapathysubramanian, Baskar
dc.contributor.author Vanous, Kimberly
dc.contributor.author Jubery, Talukder
dc.contributor.author Frei, Ursula
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Ganapathysubramanian, Baskar
dc.contributor.author Lubberstedt, Thomas
dc.contributor.department Mechanical Engineering
dc.contributor.department Agronomy
dc.contributor.department Electrical and Computer Engineering
dc.contributor.department Plant Sciences Institute
dc.date 2019-06-03T06:49:24.000
dc.date.accessioned 2020-06-30T06:05:02Z
dc.date.available 2020-06-30T06:05:02Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-04-04
dc.description.abstract <p>Potential benefits of incorporating embryo culture (EC) into a doubled haploid (DH) program, including shortening the breeding cycle and increasing chromosome doubling rates, make the laborious and tedious task of excising embryos worth the effort. Difficulties arise during embryo selection considering the marker gene <em>R1-nj</em>, which is typically used in DH programs, is not expressed in early stages after pollination. Although transgenic approaches have been implemented to bypass this issue, there is so far no known non-transgenic method of selecting haploid embryos. The findings of this study reveal methods of selecting haploid embryos that allow the possibility of incorporating EC into a DH program without using transgenic inducers. The best performing method involves a machine-learning classifier, specifically a support vector machine, which uses primary root lengths and daily growth rates as traits for classification. Selection by this method can be achieved on the third day after germination. By this method, an average false negative rate of 2% and false positive rate of 9% was achieved. Therefore, the methods presented in this research allow efficient and non-transgenic selection of haploid embryos that is simple and effective.</p>
dc.description.comments <p>This article is published as Vanous, Kimberly, Talukder Zaki Jubery, Ursula K. Frei, Baskar Ganapathysubramanian, and Thomas Lübberstedt. "Utilization of Reduced Haploid Vigor for Phenomic Discrimination of Haploid and Diploid Maize Seedlings." <em>The Plant Phenome Journal</em> 2, no. 1 (2019). DOI: <a href="http://dx.doi.org/10.2135/tppj2018.10.0008" target="_blank">10.2135/tppj2018.10.0008</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/me_pubs/348/
dc.identifier.articleid 1350
dc.identifier.contextkey 14261124
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath me_pubs/348
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/55218
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/me_pubs/348/2019_GanapathysubramanianBaskar_UtilizationReduced.pdf|||Fri Jan 14 23:42:52 UTC 2022
dc.source.uri 10.2135/tppj2018.10.0008
dc.subject.disciplines Acoustics, Dynamics, and Controls
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Mechanical Engineering
dc.subject.disciplines Plant Breeding and Genetics
dc.title Utilization of Reduced Haploid Vigor for Phenomic Discrimination of Haploid and Diploid Maize Seedlings
dc.type article
dc.type.genre article
dspace.entity.type Publication
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