Horizontal transfer generates genetic variation in an asexual pathogen

dc.contributor.author Huang, Xiaoqiu
dc.contributor.author Huang, Xiaoqiu
dc.contributor.department Computer Science
dc.date 2018-02-18T01:31:42.000
dc.date.accessioned 2020-06-30T01:54:56Z
dc.date.available 2020-06-30T01:54:56Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>There are major gaps in the understanding of how genetic variation is generated in the asexual pathogen <em>Verticillium dahliae</em>. On the one hand, <em>V. dahliae</em> is a haploid organism that reproduces clonally. On the other hand, single-nucleotide polymorphisms and chromosomal rearrangements were found between <em>V. dahliae</em> strains. Lineage-specific (LS) regions comprising about 5% of the genome are highly variable between <em>V. dahliae</em> strains. Nonetheless, it is unknown whether horizontal gene transfer plays a major role in generating genetic variation in <em>V. dahliae</em>. Here, we analyzed a previously sequenced <em>V. dahliae</em> population of nine strains from various geographical locations and hosts. We found highly homologous elements in LS regions of each strain; LS regions of <em>V. dahliae</em> strain JR2 are much richer in highly homologous elements than the core genome. In addition, we discovered, in LS regions of JR2, several structural forms of nonhomologous recombination, and two or three homologous sequence types of each form, with almost each sequence type present in an LS region of another strain. A large section of one of the forms is known to be horizontally transferred between <em>V. dahliae</em> strains. We unexpectedly found that 350 kilobases of dynamic LS regions were much more conserved than the core genome between <em>V. dahliae</em> and a closely related species (<em>V. albo-atrum</em>), suggesting that these LS regions were horizontally transferred recently. Our results support the view that genetic variation in LS regions is generated by horizontal transfer between strains, and by chromosomal reshuffling reported previously.</p>
dc.description.comments <p>This is an article from <em>PeerJ</em> 2 (2014): e650, doi :<a href="http://dx.doi.org/0.7717/peerj.650" target="_blank">10.7717/peerj.650</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cs_pubs/4/
dc.identifier.articleid 1002
dc.identifier.contextkey 9388362
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cs_pubs/4
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/19896
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cs_pubs/4/2014_Huang_HorizontalTransfer.pdf|||Sat Jan 15 00:01:14 UTC 2022
dc.source.uri 10.7717/peerj.650
dc.subject.disciplines Other Computer Sciences
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Asexual reproduction
dc.subject.keywords Horizontal transfer
dc.subject.keywords Nonhomologous recombination
dc.subject.keywords Verticillium dahliae
dc.subject.keywords Lineage-specific regions
dc.subject.keywords Genetic variation
dc.title Horizontal transfer generates genetic variation in an asexual pathogen
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication e5367231-5ba9-43f5-b3e4-e3e742211b2e
relation.isOrgUnitOfPublication f7be4eb9-d1d0-4081-859b-b15cee251456
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