Novel Observations of Peroxiredoxin-2 Profile and Protein Oxidation in Skeletal Muscle From Pigs of Differing Residual Feed Intake and Health Status

File

2021-Lonergan-NovelObservations.pdf (1.13 MB)

Date

2021

Authors

Patterson, Brian M.
Outhouse, Amanda C.
Helm, Emma T.
Johnson, Logan
Steadham, Edward M.
Dekkers, Jack C. M.
Schwartz, Kent J.
Gabler, Nicholas K.
Lonergan, Steven
Huff-Lonergan, Elisabeth

Publisher

Altmetrics

Authors

Lonergan, Steven

Person

Huff-Lonergan, Elisabeth

Person

Organizational Units

Animal Science

Organizational Unit

Veterinary Diagnostic and Production Animal Medicine

Organizational Unit

Department

Animal ScienceVeterinary Diagnostic and Production Animal Medicine

Abstract

This study’s objective was to determine the impact of a dual respiratory and enteric bacterial health challenge on the antioxidant protein peroxiredoxin-2 (Prdx-2) profile and protein oxidation in the skeletal muscle of pigs from 2 lines that were divergently selected for residual feed intake (RFI). The hypotheses were that (1) differences exist in the Prdx-2 profile between 2 RFI lines and infection status and (2) muscle from less efficient high-RFI and health-challenged pigs have greater cellular protein oxidation. Barrows (50 ± 7 kg, N = 24) from the 11th generation of the high-RFI (n = 12) and low-RFI (n = 12) Iowa State University lines were used. Pigs (n = 6 per line) were inoculated with Mycoplasma hyopneumoniae and Lawsonia intracellularis (MhLI) on day 0 post infection to induce a respiratory and enteric health challenge. Uninoculated pigs served as controls (n = 6 per line). Necropsy was at 21 d post infection. Sarcoplasmic protein oxidation, various forms of Prdx-2, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) content were determined. Neither RFI line nor infection status significantly affected protein carbonylation. Under nonreducing conditions, MhLI pigs had a greater amount of a slower-migrating GAPDH band (P = 0.017), indicating oxidative modification. Regardless of health status, the low-RFI pigs had less total Prdx-2 (P = 0.035), Prdx-2 decamer (P = 0.0007), and a higher ratio of hyperoxidized peroxiredoxin relative to Prdx-2 (P = 0.028) than the high-RFI pigs. The increased pool of active Prdx-2 in high-RFI pigs suggests greater oxidative stress in muscle in high- versus low-RFI pigs. The increase in oxidized GAPDH seen in muscle from MhLI pigs—particularly the high-RFI MhLI pigs—may be a response to the greater oxidative stress in the high-RFI MhLI. This work suggests that antioxidant proteins are important in growth and health-challenge situations.

Comments

This article is published as Patterson, B. M. & Outhouse, A. C. & Helm, E. T. & Johnson, L. & Steadham, E. M. & Dekkers, J. C. & Schwartz, K. J. & Gabler, N. K. & Lonergan, S. M. & Huff-Lonergan, E., (2021) “Novel Observations of Peroxiredoxin-2 Profile and Protein Oxidation in Skeletal Muscle From Pigs of Differing Residual Feed Intake and Health Status”, Meat and Muscle Biology 5(1), p.23, 1–15. doi: https://doi.org/10.22175/mmb.12241. Posted with permission. This is an open access article distributed under the CC BY license (https://creativecommons.org/licenses/by/4.0/).