Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines

Date
2016-10-01
Authors
Van Goor, Angelica
Slawinska, Anna
Schmidt, Carl
Lamont, Susan
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Animal Science
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Abstract

Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-γ, IL-10, IL-12β, IL-1β, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.

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This article is published as Van Goor, Angelica, Anna Slawinska, Carl J. Schmidt, and Susan J. Lamont. "Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines." Developmental & Comparative Immunology 63 (2016): 96-110. DOI: 10.1016/j.dci.2016.05.016. Posted with permission.

Keywords
Dendritic cells, Fayoumi, Leghorn, MHC-II, Nitric oxide, Gene expression
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