Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines

dc.contributor.author Van Goor, Angelica
dc.contributor.author Slawinska, Anna
dc.contributor.author Lamont, Susan
dc.contributor.author Schmidt, Carl
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2020-10-07T02:47:18.000
dc.date.accessioned 2021-02-24T21:11:44Z
dc.date.available 2021-02-24T21:11:44Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-10-01
dc.description.abstract <p>Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to <em>in vitro</em> treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes <em>BAG3</em>, <em>HSP25</em>, <em>HSPA2</em>, and <em>HSPH1</em> was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes <em>CCL4</em>, <em>CCL5</em>, <em>CD40</em>, <em>GM-CSF</em>, <em>IFN-γ</em>, <em>IL-10</em>, <em>IL-12β</em>, <em>IL-1β</em>, <em>IL-6, IL-8</em>, and <em>iNOS</em> was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.</p>
dc.description.comments <p>This article is published as Van Goor, Angelica, Anna Slawinska, Carl J. Schmidt, and Susan J. Lamont. "Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines." <em>Developmental & Comparative Immunology</em> 63 (2016): 96-110. DOI: <a href="https://doi.org/10.1016/j.dci.2016.05.016" target="_blank">10.1016/j.dci.2016.05.016</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/583/
dc.identifier.articleid 1590
dc.identifier.contextkey 19687698
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/583
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93338
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/583/2016_LamontSusan_DistinctFunctional.pdf|||Sat Jan 15 01:02:00 UTC 2022
dc.source.uri 10.1016/j.dci.2016.05.016
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords Dendritic cells
dc.subject.keywords Fayoumi
dc.subject.keywords Leghorn
dc.subject.keywords MHC-II
dc.subject.keywords Nitric oxide
dc.subject.keywords Gene expression
dc.title Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
File
Original bundle
Now showing 1 - 1 of 1
Name:
2016_LamontSusan_DistinctFunctional.pdf
Size:
2.27 MB
Format:
Adobe Portable Document Format
Description:
Collections