Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development Whitham, Steven Liu, Jian-Zhong Horstman, Heidi Braun, Edward Graham, Michelle Zhang, Chunquan Nettleton, Dan Navarre, Duroy Qiu, Wen-Li Lee, Yeunsook Nettleton, Dan Hill, John Whitham, Steven
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Statistics
dc.contributor.department Agronomy 2018-02-17T13:12:52.000 2020-06-30T06:23:52Z 2020-06-30T06:23:52Z 2011-01-01
dc.description.abstract <p>Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species such as Arabidopsis (<em>Arabidopsis thaliana</em>) and tobacco (<em>Nicotiana tabacum</em>). However, the importance of MAPK signaling pathways in the disease resistance of crops is still largely uninvestigated. To better understand the role of MAPK signaling pathways in disease resistance in soybean (<em>Glycine max</em>), 13, nine, and 10 genes encoding distinct MAPKs, MAPKKs, and MAPKKKs, respectively, were silenced using virus-induced gene silencing mediated by <em>Bean pod mottle virus</em>. Among the plants silenced for various MAPKs, MAPKKs, and MAPKKKs, those in which <em>GmMAPK4</em> homologs (<em>GmMPK4s</em>) were silenced displayed strong phenotypes including stunted stature and spontaneous cell death on the leaves and stems, the characteristic hallmarks of activated defense responses. Microarray analysis showed that genes involved in defense responses, such as those in salicylic acid (SA) signaling pathways, were significantly up-regulated in <em>GmMPK4</em>-silenced plants, whereas genes involved in growth and development, such as those in auxin signaling pathways and in cell cycle and proliferation, were significantly down-regulated. As expected, SA and hydrogen peroxide accumulation was significantly increased in <em>GmMPK4</em>-silenced plants. Accordingly, <em>GmMPK4</em>-silenced plants were more resistant to downy mildew and <em>Soybean mosaic virus</em> compared with vector control plants. Using bimolecular fluorescence complementation analysis and in vitro kinase assays, we determined that GmMKK1 and GmMKK2 might function upstream of GmMPK4. Taken together, our results indicate that GmMPK4s negatively regulate SA accumulation and defense response but positively regulate plant growth and development, and their functions are conserved across plant species.</p>
dc.description.comments <p>This article is from <em>Plant Physiology</em> 157 (2011): 1363, <a href="" target="_blank">doi:10.1104/pp.111.185686</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1077
dc.identifier.contextkey 8150804
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/75
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 01:49:09 UTC 2022
dc.source.uri 10.​1104/​pp.​111.​185686
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Pathology
dc.title Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication bd8b6e79-2efb-4611-9a27-13792f2b9a43
relation.isAuthorOfPublication 7d86677d-f28f-4ab1-8cf7-70378992f75b
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
Original bundle
Now showing 1 - 1 of 1
1.04 MB
Adobe Portable Document Format