A Review of Cry Protein Detection with Enzyme-Linked Immunosorbent Assays

dc.contributor.author Hellmich, Richard
dc.contributor.author Albright, Vurtice
dc.contributor.author Hellmich, Richard
dc.contributor.author Coats, Joel
dc.contributor.author Coats, Joel
dc.contributor.department Entomology
dc.date 2018-10-30T23:32:13.000
dc.date.accessioned 2020-06-30T02:24:40Z
dc.date.available 2020-06-30T02:24:40Z
dc.date.issued 2016-01-01
dc.description.abstract <p>The widespread use of Cry proteins in insecticide formulations and transgenic crops for insect control has led to an increased interest in the environmental fate of these proteins. Although several detection methods are available to monitor the fate of Cry proteins in the environment, enzyme-linked immunosorbent assays (ELISAs) have emerged as the preferred detection method, due to their cost-effectiveness, ease of use, and rapid results. Validation of ELISAs is necessary to ensure accurate measurements of Cry protein concentrations in the environment. Validation methodology has been extensively researched and published for the areas of sensitivity, specificity, accuracy, and precision; however, cross validation of ELISA results has been studied to a lesser extent. This review discusses the use of ELISAs for detection of Cry proteins in environmental samples and validation of ELISAs and introduces cross validation. The state of Cry protein environmental fate research is considered through a critical review of published literature to identify areas where the use of validation protocols can be improved.</p>
dc.description.comments <p>This article is published as Albright III, Vurtice C., Richard L. Hellmich, and Joel R. Coats. "A review of Cry protein detection with enzyme-linked immunosorbent assays." <em>Journal of agricultural and food chemistry</em> 64, no. 11 (2016): 2175-2189. doi: <a href="http://dx.doi.org/10.1021/acs.jafc.5b03766%20" target="_blank">10.1021/acs.jafc.5b03766 </a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/492/
dc.identifier.articleid 1492
dc.identifier.contextkey 13204586
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/492
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/24123
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/492/2016_Coats_ReviewCry.pdf|||Sat Jan 15 00:30:07 UTC 2022
dc.source.uri 10.1021/acs.jafc.5b03766
dc.subject.disciplines Agriculture
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Entomology
dc.subject.disciplines Environmental Sciences
dc.subject.disciplines Genetics
dc.subject.keywords bioassays
dc.subject.keywords cross validation
dc.subject.keywords Bt proteins
dc.subject.keywords Cry protein
dc.subject.keywords ELISA
dc.subject.keywords environmental fate
dc.subject.keywords transgenic crops
dc.title A Review of Cry Protein Detection with Enzyme-Linked Immunosorbent Assays
dc.type article
dc.type.genre article
dspace.entity.type Publication
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