Host immunity and the colon microbiota of mice infected with Citrobacter rodentium are beneficially modulated by lipid-soluble extract from late-cutting alfalfa in the early stages of infection

dc.contributor.author Fries-Craft, Krysten
dc.contributor.author Bobeck, Elizabeth
dc.contributor.author Fries-Craft, Krysten
dc.contributor.author Anast, Justin
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.author Bobeck, Elizabeth
dc.contributor.department Animal Science
dc.contributor.department Microbiology
dc.date 2020-07-21T19:44:14.000
dc.date.accessioned 2021-02-24T21:10:49Z
dc.date.available 2021-02-24T21:10:49Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.issued 2020-07-16
dc.description.abstract <p>Alfalfa is a forage legume commonly associated with ruminant livestock production that may be a potential source of health-promoting phytochemicals. Anecdotal evidence from producers suggests that later cuttings of alfalfa may be more beneficial to non-ruminants; however, published literature varies greatly in measured outcomes, supplement form, and cutting. The objective of this study was to measure body weight, average daily feed intake, host immunity, and the colon microbiota composition in mice fed hay, aqueous, and chloroform extracts of early (1st) and late (5th) cutting alfalfa before and after challenge with <em>Citrobacter rodentium</em>. Prior to inoculation, alfalfa supplementation did not have a significant impact on body weight or feed intake, but 5th cutting alfalfa was shown to improve body weight at 5- and 6-days post-infection compared to 1st cutting alfalfa (<em>P</em> = 0.02 and 0.01). Combined with the observation that both chloroform extracts improved mouse body weight compared to control diets in later stages of <em>C</em>. <em>rodentium</em> infection led to detailed analyses of the immune system and colon microbiota in mice fed 1st and 5th cutting chloroform extracts. Immediately following inoculation, 5th cutting chloroform extracts significantly reduced the relative abundance of <em>C</em>. <em>rodentium</em> (<em>P</em> = 0.02) and did not display the early lymphocyte recruitment observed in 1st cutting extract. In later timepoints, both chloroform extracts maintained lower splenic B-cell and macrophage populations while increasing the relative abundance of potentially beneficially genera such as <em>Turicibacter</em> (<em>P</em> = 0.02). At 21dpi, only 5th cutting chloroform extracts increased the relative abundance of beneficial <em>Akkermansia</em> compared to the control diet (<em>P</em> = 0.02). These results suggest that lipid soluble compounds enriched in late-cutting alfalfa modulate pathogen colonization and early immune responses to <em>Citrobacter rodentium</em>, contributing to protective effects on body weight.</p>
dc.description.comments <p>This article is published as Fries-Craft K, Anast JM, Schmitz-Esser S, Bobeck EA (2020) Host immunity and the colon microbiota of mice infected with <em>Citrobacter rodentium</em> are beneficially modulated by lipid-soluble extract from late-cutting alfalfa in the early stages of infection. PLoS ONE 15(7): e0236106. doi: <a href="https://doi.org/10.1371/journal.pone.0236106">10.1371/journal.pone.0236106</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/546/
dc.identifier.articleid 1547
dc.identifier.contextkey 18605595
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/546
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93301
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/546/2020_SchmitzEsser_HostImmunity.pdf|||Sat Jan 15 00:54:16 UTC 2022
dc.source.uri 10.1371/journal.pone.0236106
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Immunity
dc.subject.disciplines Microbiology
dc.title Host immunity and the colon microbiota of mice infected with Citrobacter rodentium are beneficially modulated by lipid-soluble extract from late-cutting alfalfa in the early stages of infection
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5cdcafbe-9e09-4a3d-9ef2-c3ab2d3cbb2b
relation.isAuthorOfPublication 1914e5d6-c767-494e-8228-448fa7aa5672
relation.isAuthorOfPublication 247d6138-be53-44be-9e88-220424e98124
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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